what is the principle behind serum protein electrophoresis
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Serum protein electrophoresis Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. Blood must first be collected, usually into an airtight vial or syringe.
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Reference Range Description. Serum protein electrophoresis (SPEP) is an easy, inexpensive method of separating proteins based on their net charge, size, and shape. The 2 major types of protein present in the serum are albumin and the globulin proteins.Albumin is the major protein component of serum and represents the largest peak that lies closest to the positive electrode.escription. Serum protein electrophoresis (SPEP) is an easy, inexpensive method of separating proteins based on their net charge, size, and shape. The 2 major types of protein present in the serum are albumin and the globulin proteins.
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- Total Serum Protein. Guide. A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. 1  Albumin is made mainly in the liver.2  It helps keep the blood from leaking out of blood vessels.otal Serum Protein. Guide. A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. 1  Albumin is made mainly in the liver.
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Diagnosis How is it used? Protein electrophoresis is used to identify the presence of abnormal proteins, to identify the absence of normal proteins, and to determine when different groups of proteins are present in unusually high or low amounts in blood or other body fluids.
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Serum Protein Electrophoresis Test What Abnormal Results for a Protein Electrophoresis Serum Test May Mean. The various proteins in the body perform different functions. This means that high or low levels of the five protein types examined during the test can point to different issues and illnesses.ne is to test for a cancer called multiple myeloma; another is to screen for multiple sclerosis, a chronic disease of the nervous system. SPEP is used to help diagnose or monitor a variety of different diseases or disorders that have abnormal proteins or protein levels.
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What is Electrophoresis | 3 Major Types & Principle in detail What is Electrophoresis & Its Principle. The term Electrophoresis means Electro=electric field + Phoresis=migration. So as the name indicates, electrophoresis is a method of separation of charged molecules in an electric field.
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Understanding and Interpreting Serum Protein Electrophoresis Serum protein electrophoresis is used to identify patients with multiple myeloma and other serum protein disorders. Electrophoresis separates proteins based on their physical properties, and the subsets of these proteins are used in interpreting the results.erum is placed on a specific medium, and a charge is applied. The net charge (positive or negative) and the size and shape of the protein commonly are used in differentiating various serum proteins. 1. Several subsets of serum protein electrophoresis are available.
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Electrophoresis Analysis Electrophoresis Analysis. Electrophoresis analysis is a method that is used to separate proteins of differing sizes and/or charges. A sample of the protein, often DNA or RNA, is placed in wells at one end of a gel.An electric current is applied that repels the proteins and causes them to move down the gel.y comparing the gel with the protein spread through it to a reference or standard sample, an identification can be made. Electrophoresis analysis is used in forensics to compare DNA, in medical laboratories to do genetic testing, and in microbiology labs to identify microorganisms.
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Original articlePrinciples and problems of the electrophoretic mobility shift assay 1. The electrophoretic mobility shift assay (EMSA) was established as a method to detect DNA binding proteins (Fried & Crothers, 1981). The principle being that a nucleic acid with protein bound, has less mobility through a gel matrix than free nucleic acid.
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What is gel electrophoresis? Electrophoresis is a technique commonly used in the lab to separate charged molecules, like DNA, according to size. Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like proteins? according to their size.
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Electrophoresis The gel may be a single concentration or gradient in which pore size decreases with migration distance. in sds gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse field electrophoresis, two fields are applied alternately at right angles to each other to minimise diffusion mediated spread of large linear polymers. See: electrofocussing, pulse field electrophoresis
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Principle of Serological Testing What method uses the principle of Ag electrophoresed into an Ab containing gel... -Specific Ab added to a agarose gel at an adjusted pH to inhibit Ab migration. -Adding Ags to a well in agarose gel. -Addition of dilutions of patient serum added to 2 Wells.
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How to Measure Protein Concentration More Accurately In general, you measure the absorbance of a series of known concentrations of a standard protein, generally BSA, and create a standard curve. You then use that standard curve to calculate the concentration of your protein sample based on its absorbance.
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- Figure 1. The standard steps in Western Blotting. Samples are prepared and loaded on to a gel and during the electrophoresis the negatively charged proteins move toward the positively charged anode. In order to further analyze the proteins, they are transferred onto a membrane in a procedure called blotting.
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Total Serum Protein  [en EspaÃ±ol] Test Overview. A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. Albumin is made mainly in the liver. It helps keep the blood from leaking out of blood vessels. Albumin also helps carry some medicines and other substances through the blood and is important for tissue growth and healing. Globulin is made up of different proteins called alpha, beta, and gamma types.
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Understanding and Interpreting Serum Protein Electrophoresis Electrophoresis is a method of separating proteins based on their physical properties. Serum is placed on a specific medium, and a charge is applied. The net charge (positive or negative) and the size and shape of the protein commonly are used in differentiating various serum proteins.1.
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Understanding and Interpreting Serum Protein Electrophoresis Serum protein electrophoresis is used to identify patients with multiple myeloma and other serum protein disorders. Electrophoresis separates proteins based on their physical properties, and the subsets of these proteins are used in interpreting the results.erum protein electrophoresis is a laboratory examination that commonly is used to identify patients with multiple myeloma and other disorders of serum protein. Many subspecialists include serum protein electrophoresis screening in the initial evaluation for numerous clinical conditions.
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Protein Solubility As the protein moves to a lower pH region, its surface charge will become less negative, and a pH region will be reached at which the protein net charge is zero (the isoelectric point). The protein will stop moving and, because different proteins have different isoelectric points, separation can be achieved.
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- Biotechnology Index Glossary Principles of Gel Electrophoresis Electrophoresis is a technique used to separate and sometimes purify macromolecules-especially proteins and nucleic acids-that differ in size, charge or conformation. As such, it is one of the most widely-used techniques in biochemistry and molecular biology.
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Medical Dictionary Why It Is Done. Serum protein electrophoresis is most often done to help diagnose and monitor a wide variety of conditions. These include: 1  Some forms of cancer. 2  Problems with the kidneys or liver. 3  Problems with the immune system. 4  Conditions that lead to poor nutrition.
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Gel electrophoresis The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted. Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
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Gel electrophoresis Gel electrophoresis. Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. Proteins can be separated according to their size and their charge (different proteins have different charges).
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Test Center Reference Range(s) Clinical Significance. Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma.
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What is the Principle, Procedure and Applications of Paper Electrophoresis? iv) Detection of Enzymes in situ: 1  If the component to be separated is an enzyme. 2  The paper strip, which have separated enzyme, is impregnated with the substrate for the enzyme desired to be separated. 3  The paper strip is now placed in a suitable buffer along with electrophoretogram.) Quantitative estimation: The color density of the zone may be multiplied with the area of the zone and the resulting value would be a rough estimate of the concentration of the component. Paper Electrophoresis is one of the zone electrophoresis. This is very important method in all laboratories.
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- Serum albumin is one of the most widely studied proteins and is the most abundant protein in plasma with a typical concentration of 5g/100ml.he BSA molecule is made up of three homologous domains (I, II, III) which are divided into nine loops (L1-L9) by 17 disulphide bonds. The loops in each domain are made up of a sequence of large-small-large loops forming a triplet. Each domain in turn is the product of two subdomains (IA, IB, etc.).