importance of biochemical tests in identification of bacteria
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Importance of Biochemical Tests of Bacteria Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities of different bacteria.
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- A biochemical assay is an analytical in vitro procedure used to detect, quantify and/or study the binding or activity of a biological molecule, such as an enzyme.
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Why is identifying Bacteria important? The bacteria also eat away the chemicals in the body of organism it infects and break down the chemicals. Various groups of bacteria carry out different processes in the body to generate symptoms of the disease. So, it is necessary to identify the bacteria and find out to which class it belongs to.ome bacteria are aerobic and some are anaerobic. Some travel in air, some in water and some through any object from one hand to the hand of other people. They travel in the medium with the help of flagella or by wriggling.
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Summary of Biochemical Tests The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria. The differential ingredient is lactose. Fermentation of this sugar results in an acidic pH and causes the pH indicator, neutral red, to turn a bright pinky-red color. Thus organisms capable of lactose fermentation such as Escherichia coli, form bright pinky-red colonies (plate pictured on the left here).
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- Clinical Microbiology-This section of the lab is involved with a variety of biological specimens such as: urine, feces, blood, sputum, CSF, drainage, exudates, nail, skin, tissue, and swabs from throat, wounds, and other such specimens.
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- Gram Stain The Gram stain is the most important staining procedure in microbiology. It is used to differentiate between gram positive organisms and gram negative organisms.
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Importance of Biochemical Tests of Bacteria A list of some of the most commonly used biochemical tests performed for the identification of bacteria has been given in Figure 7.1. Each species of bacteria has a well-defined set of metabolic activities different from all other species.
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Useful For GRAM : The Gram stain is a general stain used extensively in microbiology for the preliminary differentiation of microbiological organisms. The Gram stain is one of the simplest, least expensive, and most useful of the rapid methods used to identify and classify bacteria.
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- Three Parts:Preparing the SlidePerforming the Gram StainExamining the ResultCommunity Q&A. Gram staining is a quick procedure used to look for the presence of bacteria in tissue samples and to characterise bacteria as Gram-positive or Gram-negative, based on the chemical and physical properties of their cell walls.
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- There may be occasions when food microbiology laboratories need to test food samples for the presence of bacterial toxins, for example in the investigation of cases of food poisoning, or if a process failure has occurred.This is unlikely to be a routine requirement for most industrial laboratories.ngestion of food containing at least 0.1-1 μg of toxin can cause a mild form of food poisoning with a rapid onset of symptoms (2-4 hours), including nausea and vomiting with abdominal cramps, occasionally followed by diarrhoea. Recovery is usually completed within 48 hours and deaths are very rare.
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- Tennis – The Championships Wimbledon: Gentlemen's Singles Trophy, first presented by the All England Club in 1887, is made of silver gilt, stands 18 inches high, and has a diameter of 7.5 inches. The Ladies’ Singles Trophy, a silver salver, was first presented in 1886.riginally called the Dominion Hockey Challenge Cup, the trophy was a decorative punch bowl, measuring 18.5 by 29.0 centimeters, made of silver by famed London silversmith G. R. Collis and Co. With the advent of professional sports leagues, the trophy became the Presentation Cup in 1926.
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- Cosmetics are often used as samples for microbial tests. A microbial test can be used to find sources of contamination. Microbial tests involve use of a lab. Microbial tests check for the presence of potentially harmful micro-organisms in a sample. Labs actively cultivating micro-organisms in a petri dish when conducting microbial tests.
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- A Gram stain is a lab test used to detect bacteria or fungi in a sample taken from the site of a suspected infection. It gives relatively quick, preliminary results as to whether microbes are present and, if so, the general type(s) causing an infection.
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- In many distinct areas of microbiology, the ability to identify microorganisms has important application. For example, in food microbiology it is important to be able to accurately identify food spoilage contaminants. In microbial ecology, the identification of microorganisms helps us characterize biodiversity.
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- A stool Gram stain is a laboratory test that uses different stains to detect and identify bacteria in a stool sample. The Gram stain method is sometimes used to quickly diagnose bacterial infections. News & Features. Pink and Purple Germs.
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Classification of Bacteria The classification of bacteria of medical importance has been carried out according to the 'Gram reaction' all these years. Gram reaction is a medical staining test named after Christian Gram who came up with the process in 1884.
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Biochemical Reagents for Identification Biochemical Reagents for Identification. A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria. Classical biochemical tests are often used to identify microorganisms; the results are seen by color change. In most cases, detection is based on the reaction of an enzyme with a certain substrate. Also, methods to detect certain metabolites by chemical reaction and or complex building techniques are used.
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- Laboratory tests. Examples of common tests include: Bacterial culture – This is the primary test used to diagnose a bacterial infection. Results are usually available within 24-48 hours. Gram stain – This is usually performed in conjunction with the wound culture. It is a special staining procedure that allows bacteria to be evaluated under the microscope.
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- These tests detect the presence of disease-causing (pathogenic) Escherichia coli that produce Shiga toxins. E. coli bacteria commonly occur in nature and they are a necessary component of the digestive process in humans and most other mammals.
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- : The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.he traditional culture and colony counting method has been a practical for the detection and identification of pathogens in food, including microbiological culturing and isolation of the pathogen, followed by confirmation by biochemical and serological tests, which takes up to 5–7 days to get a result [ 11 ].
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- The inadvertent use of a mixed culture in biochemical testing will lead to erroneous results. The simplest biochemical test that is used to differentiate between Streptococcus and Staphylococcus species is the Catalase test.
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Summary of Biochemical Tests Blood Agar Plates (BAP). This is a differential medium. It is a rich, complex medium that contains 5% sheep red blood cells. BAP tests the ability of an organism to produce hemolysins, enzymes that damage/lyse red blood cells (erythrocytes).
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What is the principle and procedure of gram staining? Gram staining is a widely used and most essential microbiology technique. It is used to identify bacteria by differentiating between Gram positive and Gram negative bacteria. It also aids taxonomic divisions of bacteria. The procedure differentiate the bacteria based on the composition of their cell wall.
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- An antibiogram is the result of a laboratory testing for the sensitivity of an isolated bacterial strain to different antibiotics. It is by definition an in vitro-sensitivity.
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Biochemical Tests The catalse test is primarily used for gram positive bacteria and can for instance be utilized to distinguish Staphylococcus spp. and Micrococcus spp., which are catalase positive from Streptococcus spp. and Enterococcus spp., respectively, which are catalase negative. Updated: 2013-11-19.
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- Asian J. Med. Sci., 3(5): 206-217, 2011 209histopathology with vibriosis and pseudomonas infection,but can be differentiated with the identification of thebacterial organisms, using biochemical tests.In the treatment and control of the disease, all kindsof stress factors should be avoided.