Purpose The tumour suppressor protein RB plays a decisive role in negative control of the cell cycle  inhibiting tumour development The present analysis investigated the prevalence of the nucleotide polymorphism A153104G  which is located at intron 18 of the RB1 gene  and investigated the impact of the polymorphic variability in the exon 19 and its flanking intronic sequences on the severity of cervical disease in HPV16-positive Greek women