Isozyme patterns of 13 enzymes were compared for cultures of Trypanosoma avium  T vespertilionis  T cruzi and T rangeli The isozyme separation was made by cellulose acetate electrophoresis Each of the species had distinctly migrating isozyme bands for glutamate oxaloacetate transaminase (GOT)  isocitrate dehydrogenase (ICD)  malic enzyme (ME)  6-phosphogluconate dehydrogenase (6PGD)  phosphoglucoisomerase (PGI)  phosphoglucomutase (PGM)  and malic dehydrogenase (MDH)