What is the difference between the use of Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE other than the volume of NaOH or HCL needed for the pH adjust?
I need to make a pH 7.0 phosphate buffer, but I am missing KH2PO4. I have K2HPO4, can I just use HCl to bring the pH down to 7.0 to get the desired buffering capacity?