aakash kabbin
Senior Research Associate at Cellarity
senior research associate hematology , ipsc
stem cell biologist , look within body turn fundamental , seek answer disease . explore avenue far reaching effect , guided intuition developed observing biological phenomenon happening around .
Cellarity
https://www.linkedin.com/company/cellarity
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Senior Research Associate
CRISPR Therapeutics
https://www.linkedin.com/company/crispr-therapeutics
Gene Therapy for β-Hemoglobinopathy –
•	Designed and executed various CRISPR gene editing strategies to increase fetal hemoglobin levels in vitro in Hematopoietic Stem Cells (HSCs). 
•	Supported various clinical drug candidate (for Sickle Cell Disease (SCD) and β-thalassemia) related activities including in vitro erythroid differentiation assay, colony forming unit assay, mouse xenotransplantation, flow cytometry, molecular assays to assess gene editing frequency and indel patterns. 
•	Assume leadership roles within the research organization including writing multiple protocols, scientific reports, taking responsibility for a tissue culture room supplies and serving as the emergency contact for several freezers.

HSC Platform –
•	Planned and implemented experiments to increase editing and deletion efficiencies in CD34+ cells and to increase CD34+ cell engraftment in mice models using small molecule inhibitors. 
•	Testing different gene editing, media conditions and small molecule approaches to maintain and proliferate CD34+ cells in culture. 
•	Working with in vivo team to study HSC subpopulation’s (based on cell surface markers) colony forming and engraftment potential. 
•	Collaborating with bioinformatics team on HSC single cell RNAseq experiment to look for markers responsible for long term HSC maintenance and proliferation.   

Disease modelling - Myotonic Dystrophy using iPSCs
•	Successfully developed a protocol to differentiate induced pluripotent stem cells (iPSCs) into cardiomyocytes. 
•	Established a flow cytometry assay to tests for maturation of cardiomyocytes.
Greater Boston Area
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Senior Research Associate
BERG Health
https://www.linkedin.com/company/berghealth
Parkinson's Disease Modeling using iPSCs.

- Differentiated induced pluripotent stem cells (iPSCs) from patient, knock-out and reporter lines into cortical and dopaminergic neurons. 
- Conducted Western Blots to analyse protein expression in all cell lines. 
- Will be involved in designed gRNA for CRISPR mediated gene editing to generate PIG3 knock out lines.
- Will be involved in troubleshooting dopaminergic differentiation protocol.
Greater Boston Area
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Research Associate II - Stem Cell Biology/Neurology
David Geffen School of Medicine at UCLA
https://www.linkedin.com/company/david-geffen-school-of-medicine-at-ucla
Gene Therapy for Sickle Cell Anemia:
- Using CRISPR technology along with AAV donors, corrected Sickle Cell mutation in Erythroid Progenitor cells. 
- Optimized CD34+ cell electroporation protocols on MaxCyte® Electroporator as a part of scaling up for pre-clinical trials. 
- Conducted Erythroid differentiation in PBSCs using various media and protocols and varying time points to access for the best protocol. 
- Analysed for different haemoglobin expression levels in all of the above cell types using HPLC.

Skills Acquired:
Electroporation, Transfections, CRISPR-Cas9 Gene Editing, Flowcytometry
Greater Los Angeles Area
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Staff Research Associate - Kohn Lab
Keck School of Medicine of the University of Southern California
https://www.linkedin.com/company/keckschoolusc
Amyotrophic Lateral Sclerosis (ALS) Disease Modeling:
- Differentiated induced Pluripotent Stem Cells (iPSCs) from patients, control, reporter, knock-out and isogenic lines into motor neurons (iMNs).
- Determined the most favorable conditions to co-cultured these iMNs with muscle cells.
- Performed immune staining over time to monitor the progression of ALS. 
- Once this disease model is established the next step will be to conduct drug screening. 

Generation of C9Orf72 Knock-out lines:
- Assisted in designing guide RNA for regions flanking the C9Orf73 exons and made incisions using CRISPR.
- Designed primers for the flanking regions and performed genotyping using PCR and PAGE. 
- Performed clonal purification on the positive hits by growing them on mouse embryonic fibroblast (MEFs)
- Generated a new C9Orf72 KO line. 

Skills Acquired:
PCR, Transfection, Immuno-staining, Clonal Purification, PAGE, Primer Designing, Confocal Microscopy
Greater Los Angeles Area
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Graduate Student Research Assistant - Ichida Lab
The New York Stem Cell Foundation
https://www.linkedin.com/company/nyscf
Blood Reprogramming:
- Isolated and cultured Peripheral Blood Mononuclear Cells (PBMCs) from whole blood. 
- Successfully reprogrammed PBMCs into induced Pluripotent Stem Cells (iPSCs) using Sendai Virus (SeV)
- Determined the optimum condition and time frame to deactivate Sendai Virus after reprogramming. 
- Further help optimize the SeV deactivation protocol used then at NYSCF. 
- This data provides a critical solution for reprogramming somatic cells without the presence of SeV backbone and accelerate the clinical application.

Cell Fate Mapping of PBMCs:
- Performed cell fate mapping of PBMCs in suspension and adherent cultures.
- Analyzed the two different cell population using FACS.
- Determined the best culture condition for PBMCs. 
- This data reveals that suspension culture yield higher amount of reprogrammable PBMCs and there by improves the blood reprogramming efficiency.

Neuronal Differentiation
- Differentiated iPSCs into neural progenitor cells. 
- Conducted immune-cyto chemistry and studied their morphology.

Skills Acquired:
FACS, PCR, Fluorescent Microscopy, Immuno-Staining, Transfection, Colony Picking, Suspension Culture, Cryopreservation
Greater New York City Area
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Research Intern
StemRx BioScience Solutions - India
https://www.linkedin.com/company/stemrx-bioscience-solutions---india
Screening for Cytotoxicity and Neuro-protective effects of Herbal extracts on Mesenchymal Stem Cells:
- Followed procedures and completed adequate trainings required for working in Biosafety Level 3 Lab.
- Isolated Mesenchymal Stem Cells (MSCs) from human umbilical cord tissue.
- Characterized these MSCs using stem cell markers and studied their proliferation rates.
- Determined toxicity of herbal extracts on these MSCs. 
- Examined cellular differentiation of MSCs in-vitro using herbal extracts. 
- Designed clinical trials using the data gathered from the above project and formulated herbal tablets for patients suffering from neuro-degenerative disease.

Skills Acquired:
Biosafety Level 3 Training, Primary Culture, Cytotoxicity Assay, Differential Staining, Tissue Isolation.
Mumbai Area, India
Research Intern
Analytical Solutions - A Division of Indtech Instruments Pvt. Ltd.
https://www.linkedin.com/company/analytical-solutions---a-division-of-indtech-instruments-pvt-ltd-
Pharmaceutical Formulation:
- Isolated polyphenols from Indian medicinal herbs by aqueous and ethanol extraction.
- Performed HPLC analysis for determining polyphenolic content in the extracts.
- Formulated tablets using herbal extracts and designed clinical trial for patients suffering from neurodegenerative diseases.

Skills Acquired:
HPLC, Tablet Manufacturing, Drug Formulation, Soxhlet Extraction.
Mumbai Area, India
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Research Intern
Master of Science (M.S.)
Stem Cell Biology and Regenerative Medicine
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University of Southern California
https://www.linkedin.com/school/university-of-southern-california/
Master of Science (M.S.)
Biotechnology
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Northeastern University
https://www.linkedin.com/school/northeastern-university/
Bachelor of Technology (B.Tech.)
Biotechnology
D.Y. Patil University, School of Biotechnology and Bioinformatics
Science
SIES - College of Arts, Science and Commerce
Advanced Analytical Techniques
BBT 405
Animal Biotechnology
BBT 604
Bioinformatics - I (Principles)
BBT 506
Bioinformatics - II
BBT 606
Biological and Biochemical Techniques
BBT 303
Biophysics
BBT 306
Bioprocess Technology
BBT 603
Biostatistics
BBT 206
Biotechnology Management
BBT 702
Cell Biology
BBT 101
Commercial Aspects of Biotechnology
BBT 503
Developmental Biology
BBT 204
Down Stream Processing
BBT 703
Environmental Biotechnology
BBT 602
Enzymology and Enzyme Technology
BBT 402
Genetic Engineering - I (Principles)
BBT 505
Genetic Engineering - II (Applications)
BBT 605
High Throughput Research Tools
BBT 705
IPR, Biosafety, Bioethics and Entrepreneurship
BBT 704
Immunology and Virology
BBT 406
Intermediary Metabolism - I
BBT 401
Intermediary Metabolism - II
BBT 502
Mammalian Anatomy
BBT 304
Mammalian Physiology
BBT 305
Microbiology
BBT 301
Molecular Biology of Eukaryotes
BBT 404
Molecular Biology of Prokaryotes
BBT 403
Molecular Cell Signaling
BBT 502
Molecules of Life
BBT 302
Neurobiology and Behavioral Sciences
BBT 601
Plant Biotechnology
BBT 504
Principles of Drug Action, Modeling and Design
BBT 701
Research Methodology and Scientific Writing
BBT 706
Biological Imaging in Stem Cells
SCRM 522
Bringing Stem Cells to the Clinic
SCRM 515
Culture and Differentiation of Human Pluripotent Stem Cells
SCRM 524
Current Topics in Regenerative Medicine
DSR 610
Current Topics in Stem Cell Biology and Organogenesis
DSR 620
Developmental Biology and Human Embryology
SCRM 511
Stem Cell and Developmental Biology Seminar Series
DSR 574
Stem Cells and Regenerative Medicine
SCRM 513
Writing about Stem Cell Biology and Regenerative Medicine
SCRM 555
Cell Culture Process/Biopharma production
BIOT 5613
Experimental Design/Biostatistics
PHSC 6214
Introduction to Biotechnology
BIOT 5120
Molecular Cell Biology for Biotechnology
BIOL 6299
Protein Chemistry
CHEM 5620
Stem Cells and Regeneration
BIOL 5543
The Biotechnology Enterprise
BIOT 5219
Examined the financial performance and business operations of Ariad Pharmaceuticals, and recommended strategies to raise funding capital, improve revenue generation and increase global market presence in unexplored markets.
Strategic Consulting for Ariad Pharmaceuticals
Education
Keck School of Medicine of the University of Southern California
https://www.linkedin.com/company/keckschoolusc
Stem Cell Awareness Day:

- Assisted in overall preparation of the event.
- Guided high school student teams through the Stem Cell Labs at the Broad CIRM Center. 
- Educated these students on Stem Cell thereby creating awareness amongst them.
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Education Volunteer
Arts and Culture
D.Y. Patil University
Marketing of College Cultural Festival 

- Managed a team of 25 volunteers for marketing and fundraising for the annual cultural event 'Species'.
- Approached 7 educational institutes for the promotion of the event.
- Approached 9 companies for sponsorship for marketing and hospitality for event.
- Generated a small amount of money for the event and brought in students from other educational institute for the event.
Marketing Volunteer
Animal Welfare
Turtle Conservation project, Orissa, India
Turtle Conservation Project, Orissa, India. 

- Assisted the Chief Conservationist in rescuing turtles during the hatching season.
- Patrolled the coastline to keep away predators and clean washed off nets and debris.
- Raised awareness for conservation of turtles amongst people living by the coast.
Community Volunteer
Thermo Fisher Scientific
24 HOURS OF STEM CELL
I2IT
Molecular Biology and Bioinformatics Workshop
National Burns Center - India
National Symposium on Stem Cell and Regeneration
Haffkine Institute
Enzymology and Molecular Biology Training
Haffkine Institute
Medical Virology Workshop
https://www.linkedin.com/in/joseph-nneji-66a66695
Greater Boston
Joseph Nneji
Senior Research Associate at Cellarity
https://www.linkedin.com/in/emilie-artru-17b2bab2
Boston, MA
Emilie Artru
Research Associate at Cellarity
https://www.linkedin.com/in/orna-lynch-535739129
Boston, MA
Orna Lynch
Research Associate II at Cellarity
https://www.linkedin.com/in/xiaofengjiang
Boston, MA
Xiaofeng Jiang
Senior Scientist, Cellarity
https://www.linkedin.com/in/an-249474106
Boston, MA
Atiq Nurani
Research Associate at Cellarity
https://www.linkedin.com/in/laura-isacco-042199a4
Boston, MA
Laura Isacco
Supervisor, Data Generation at Cellarity
https://www.linkedin.com/in/olivier-bezy-a42a9031
Boston, MA
olivier bezy
Senior Director - Head of Exploratory at Cellarity
https://www.linkedin.com/in/chad-nusbaum-4719b3a2
Cambridge, MA
Chad Nusbaum
Chief Technology Officer at Cellarity, Inc
https://www.linkedin.com/in/kevin-michael-henry-7a5a6812a
Cambridge, MA
Kevin Michael Henry
VP, Head of Legal & Intellectual Property
https://www.linkedin.com/in/isabelle-le-mercier-5975a320
Cambridge, MA
Isabelle LE MERCIER
Principal Scientist, Immuno Oncology at Cellarity
https://www.linkedin.com/in/nassim-ajami-9684b53b
Cambridge, MA
Nassim Ajami
Data Science, Bioinformatics, Systems Biology
https://www.linkedin.com/in/aditya-khedkar-a739b066
San Francisco, CA
Aditya Khedkar
Principal Research Associate at CRISPR Therapeutics
https://www.linkedin.com/in/jorge-buendia-buendia
Cambridge, MA
Jorge Buendia-Buendia
Scientist, Computational Biology at Cellarity
https://www.linkedin.com/in/winnie-lee-10454a14
Greater Boston
Winnie Lee
Associate Scientist
https://www.linkedin.com/in/sylvia-andrzejewski-1b875958
Boston, MA
Sylvia Andrzejewski
Senior Scientist
https://www.linkedin.com/in/smitha-krishnan-592b9a64
Greater Boston
Smitha Krishnan
Senior Scientist I (Liver Diseases), Prime Medicine
https://www.linkedin.com/in/pooja-smruthi-keerthipati
San Francisco Bay Area
Pooja Smruthi Keerthipati
CRISPR Therapeutics
https://www.linkedin.com/in/varshaneelakantan
San Francisco, CA
Varsha Neelakantan
SRA I, Functional Genomics - Maze Therapeutics
https://www.linkedin.com/in/khushboo-undavia
Greater Boston
Khushboo Undavia
Associate Scientist at Editas Medicine
Liked by Aakash Kabbin
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Happy New Year from Cellarity! We wish everyone a healthy and happy 2022. #HappyNewYear2022
Liked by Aakash Kabbin
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To every #Scientist who ran experiments as part of the COVID-19 vaccine effort!To every Individual who volunteered to be part of the COVID-19…
Liked by Aakash Kabbin
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At Vor, we are fortunate for the educational opportunities we have been afforded to build the scientific expertise needed to tackle the biggest…
CRISPR Forum
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The American Society for Cell Biology - ASCB
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Cell Therapy Industry group
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California Biotech
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Biotechnology (2091382)
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Boston Biotech
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myBIO Community - Biotechnology connections
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Stem Cell Research
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India Biotechnology & Pharmaceutical Association
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California Institute for Regenerative Medicine (CIRM)
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Pharma / Biotech, Digital Health and Life Sciences Consulting and Investment
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D.Y.Patil university Alumni USA
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Biotech India - maintained by ABLE
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BioSpace Biotechnology and Pharmaceutical Professionals Group
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