aaron mcclelland
Scientist I at NanoString Technologies, Inc.
scientist nanostring technology , inc .
passionate , innately curious forever analytical . academic biomedical researcher extensive microrna experience drive acquire new skill addition honing already acquired . strong background diabetes , kidney , microrna gene delivery . excels hypothesis building , project planning management multiple research stream .
NanoString Technologies, Inc.
https://www.linkedin.com/company/nanostring-technologies
Seattle, Washington, United States
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Scientist I
University of Washington
https://www.linkedin.com/company/university-of-washington
Studied the effects of aging and also focal segmental glomerulosclerosis on kidney health. Work was specifically focused on granular juxtaglomerular cells and their ability to migrate to sites of podocyte loss where these cells undergo lineage reprogramming/transdifferentiation, effectively replacing lost podocytes and restoring nephron function.
•	Establish primary and conditionally immortalized cell lines from renal tissue and bone.
•	Isolate cell populations through differential sieving, selective media and cloning rings.
•	Perform lineage reprogramming on primary and conditionally immortalized cell lines.
•	Utilize FACS to isolate transgenic cells from experimental animals.
•	Amplify RNA from FACS populations for qRT-PCR, NGS library preparation and microarray.
•	Conduct multiplexed immunofluorescence on cell monolayers and mounted tissue sections.
•	Create Java scripts for pre-processing of confocal images with ImageJ.
•	Write R scripts to conduct GO, GSEA and SPIA/GAGE analysis of microarray and NGS data.
•	Predict gene targets using custom data integration of multiple public datasets using R. 
•	Validate gene targets via qRTPCR and western blot.
•	Perform visual data dimensionality reduction using CIRCOS (Perl) and SOM (R).
•	Assist in mouse uninephrectomy procedures.
•	Perform follow-up care on uninephrectomized mice.
•	Manage aging experimental murine cohorts to ensure correct study size.
•	Euthanize murine subjects via intraperitoneal injection or cervical dislocation according to IACUC standards.
•	Wrote, edited and contributed to peer-reviewed journal articles.
•	Contributed to slide decks for national and international scientific meetings.
Seattle, Washington, United States
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Postdoctoral Researcher
Monash University
https://www.linkedin.com/company/monash-university
Performed data entry operations on behalf of the Aspirin in Reducing Events in the Elderly (ASPREE) trial. 
•	Confirm study questionnaire completion.
•	Validate accuracy of machine-entered documents against hard copies.
•	Correct and update machine-entered database record discrepancies.
•	Maintain study subject confidentiality.
•	File study questionnaires accordingly.
Melbourne, Victoria, Australia
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Data Entry Officer ASPREE study
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Aid in the study of novel anti-inflammatory lipoxins and their role in reducing diabetes-induced kidney disease and atherosclerosis.
•	Isolate total RNA from human and mouse urinary exosome.
•	Conduct necropsy of heart and abdominal and thoracic aorta.
•	Clean and pin full length aorta and assess atherosclerosis via oil-red-O histochemistry.
•	Process, mount and section frozen and paraffin embedded necropsy tissues.
•	Perform and quantitate immunofluorescent and immunohistochemical staining.
•	Ascetically prepare and deliver novel pharmaceuticals to experimental animals via intraperitoneal injection.
•	Carry out post-injection monitoring of treated experimental animals.
Melbourne, Victoria, Australia
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Research Associate
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Studied the effect of microRNA in the onset and advancement of diabetes-induced kidney fibrosis. Work was specifically focused on the interplay of microRNA-21 and TGFβ in proximal tubule epithelial cells and how these factors influence induction of epithelial-to-mesenchymal transition. Multiple targets were bioinformatically identified and their contributions to cellular dysfunction delineated in canonical and non-canonical TGFβ signaling. Non-canonical modes of fibrotic induction, including mitochondrial dysfunction were also investigated.
•	Modify gene expression through transfection of oligonucleotides and gene expression vectors.
•	Measure growth factors secreted in culture media via ELISA.
•	Assess cellular respiration and mitochondrial dynamics in response to microRNA/growth factors via western blot, 2D/3D confocal microscopy, multicolor flow cytometry and Seahorse XF.
•	Demonstrate correlation between localized microRNA expression levels, tissue pathology and human disease.
•	Integrate microRNA and mRNA NGS data with KEGG pathways to generate custom networks.
•	Bioinformatically identify and validate novel mitochondria associated microRNA targets.
•	Design 3’ UTR constructs and utilize heat competent E.coli to generate luciferase expression vectors.
•	Delineate the contribution multiple microRNA targets to fibrotic TGFβ signaling. 
•	Design, sub-clone, assemble and deliver shRNA-GFP and 3’UTR-GFP AAV in vivo.
•	Perform ultrasound microbubble-mediated delivery of shRNA expression vectors in vivo.
•	Train and guide junior lab members in lab skills.
•	Present research at international conferences and regularly give seminars at internal meetings.
•	Write, edit and contribute to peer-reviewed journal publications, book chapters and seminar presentations.
•	Execute material transfer agreements for novel cell lines and small molecule inhibitors.
•	Collaborate internationally to gain access to human biopsy material and aid in development of novel pharmaceuticals.
Melbourne, Australia
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PHD Graduate Student
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Aid in collecting tissues from experimental mice for a variety projects concerned with diabetes-induced kidney disease, heart disease, atherosclerosis and oxidative stress.
•	Perform necropsy of all major organs including abdominal and thoracic aorta.
•	Embed fresh tissues in optimal cutting temperature media in preparation for cryosectioning.
•	Dissect tissues accordingly and fix in prepared paraformaldehyde for paraffin embedding by external services.
•	Utilize phenol extraction to purify RNA from human pancreatic biopsies.
•	Amplify pancreatic RNA samples and assess mRNA and microRNA expression.
Melbourne, Victoria, Australia
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Technical Associate
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Assessed the role of microRNA-21 in diabetes-induced proximal tubule epithelial cell-mediated kidney fibrosis with a particular focus on epithelial-to-mesenchymal transition.
•	Designed, generated and subcloned gene expression vectors utilizing transformed E.coli and transfected mammalian cells for use in adenoviral (AAV), lentiviral and conventional transfection and transduction gene delivery systems. 
•	Transfected microRNA mimics and nucleic acid inhibitors to demonstrate microRNA roles in growth factor induced gene expression. 
•	Analyzed secreted growth factors in cell culture media by enzyme-linked immunosorbent assay (ELISA).
•	Harvested and prepared mouse tissues for further analysis including RNA analysis, protein extraction and histological assessment.
•	Trained incoming lab members (graduate students, MD PhDs, visiting academics) in basic laboratory skills.
•	Presented research at local research conferences and regularly gave seminars at internal meetings.
Melbourne, Australia
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Honors Research Student
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
•	Generate heat competent E.coli.
•	Establish primary cell lines from renal tissue via density gradient centrifugation.
•	Aid in development of bovine serum albumin-based RNA harvesting protocol.
Melbourne, Victoria, Australia
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Technical Associate
Mater Research
https://www.linkedin.com/company/mater-research
Conducted gene promoter assays as part of a larger project concerned with the major isoforms of Receptor for Advanced Glycation End products (RAGE) and their contribution to diabetes-induced kidney disease.
•	Sub-clone gene promoters to luciferase expression vectors.
•	Bulk generate sub-cloned expression vectors utilizing heat competent E.coli.
•	Conduct gene promoter assays with co-transfected gene product vectors.
•	Consult with external stakeholders to determine project objectives.
•	Relay project findings and implications for parent project to external stakeholders.
Brisbane, Queensland, Australia
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Research Associate
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Assisted principal investigators in designing and sub-cloning gene expression vectors and assessing microRNA activity in a variety of kidney cell types in the setting of diabetes. 
•	Design and sub-clone multiple 3’ UTR luciferase gene expression vectors.
•	Conduct luciferase assays to assess microRNA binding and translational repression of expression constructs.
•	Assess microRNA targets in vitro via qRTPCR and western blot of target genes.
• Routinely performed RNA and protein extraction from in vitro samples.
• Designed and subcloned 3’ UTR luciferase expression vectors utilizing E.coli.
• Created buffers and other standard laboratory reagents according to required specifications and laboratory standards.
• Gained familiarity with qPCR, western blot, FACS, mammalian cell culture, bacterial cell culture, sterile technique, gene delivery (transduction, transfection and transformation).
Melbourne, Victoria, Australia
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Summer Intern
Baker Heart and Diabetes Institute
https://www.linkedin.com/company/baker-heart-and-diabetes-institute
Assist principal investigators with the design and construction of lentiviral gene expression vectors, the assembly of virus particles and the establishment of lentivirus transduced cell lines.
•	Aid design of a tetracyclin activated microRNA lentiviral expression vector containing an IRES controlled GFP gene.
•	Aid in sub-cloning of expression vector components and transform E.coli via electroporation
•	Conduct lentiviral packaging utilizing 293T cells.
•	Perform dual resistance marker clonal selection of transformed E.coli.
•	Conduct clonal selection of lentivirus transduced cell lines and confirm transgene activity by qRTPC and fluorescent microscopy.
Melbourne, Victoria, Australia
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Summer Intern
Doctor of Philosophy - PhD
Thesis title: "microRNA-21 in Diabetic Nephropathy and a Cross-Sectional Analysis of
the Role of TGFβ in Proximal Tubule Epithelial Cells and Mesangial Cells"
Biochemistry and Molecular Biology
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Monash University
https://www.linkedin.com/school/monash-university/
Bachelor of Science Honors
High Distinction (4.0 GPA)
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Monash University
https://www.linkedin.com/school/monash-university/
Bachelor of Biotechnoloy and Molecular Cell Biology
Biochemistry and Molecular Biology
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La Trobe University
https://www.linkedin.com/school/la-trobe-university/
Nuclear Expression and DNA Binding Capacity of Receptor for Advanced Glycation End Products in Renal Tissue
bioRxiv
https://www.biorxiv.org/content/10.1101/632596v1
Lipoxins Protect Against Inflammation in Diabetes-Associated Atherosclerosis
Diabetes
https://diabetes.diabetesjournals.org/content/67/12/2657
Cells of NG2 lineage increase in glomeruli of mice following podocyte depletion
American Journal of Physiology: Renal physiology
https://journals.physiology.org/doi/full/10.1152/ajprenal.00118.2018
Lipoxins Regulate the Early Growth Response–1 Network and Reverse Diabetic Kidney Disease
Journal of the American Society of Nephrology
https://jasn.asnjournals.org/content/29/5/1437
RAGE Deletion Confers Renoprotection by Reducing Responsiveness to Transforming Growth Factor-β and Increasing Resistance to Apoptosis
Diabetes
https://diabetes.diabetesjournals.org/content/67/5/960
Sex differences in transcriptomic profiles in aged kidney cells of renin lineage
Aging
https://www.aging-us.com/article/101416/text
Charting the transcriptional landscape of cells of renin lineage following podocyte depletion
PLoS One
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189084
MicroRNAs as Biomarkers of Diabetic Nephropathy
Springer Reference
https://link.springer.com/referenceworkentry/10.1007%2F978-94-007-7699-9_18
miRNAs in the Pathophysiology of Diabetes and Their Value as Biomarkers
Academic Press
https://www.sciencedirect.com/science/article/pii/B9780128018996000310?via%3Dihub
miR-21 promotes renal fibrosis in diabetic nephropathy by targeting PTEN and SMAD7
Clinical Science
https://portlandpress.com/clinsci/article-abstract/129/12/1237/71932/miR-21-promotes-renal-fibrosis-in-diabetic?redirectedFrom=fulltext
Study of microRNA in diabetic nephropathy: Isolation, quantification and biological function
Nephrology
https://onlinelibrary.wiley.com/doi/full/10.1111/nep.12374
Transcriptome-Based Analysis of Kidney Gene Expression Changes Associated with Diabetes in OVE26 Mice, in the Presence and Absence of Losartan Treatment
PLoS One
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096987
MicroRNA in diabetic nephropathy: Renin angiotensin, AGE/RAGE, and oxidative stress pathway
Journal of Diabetes Research
https://www.hindawi.com/journals/jdr/2013/173783/
MicroRNA in the development of diabetic complications
Clinical Science
https://portlandpress.com/clinsci/article-abstract/126/2/95/69260/microRNA-in-the-development-of-diabetic?redirectedFrom=fulltext
Where are we in diabetic nephropathy: MicroRNAs and biomarkers
Nephrology and Hypertension
https://insights.ovid.com/article/00041552-201401000-00013
Transforming growth factor-β1-mediated renal fibrosis is dependent on the regulation of transforming growth factor receptor 1 expression by let-7b
Kidney International
https://www.kidney-international.org/article/S0085-2538(15)56199-1/fulltext
miR-200a Prevents Renal Fibrogenesis Through Repression of TGF-2 Expression
Diabetes
https://diabetes.diabetesjournals.org/content/60/1/280
https://www.linkedin.com/in/david-scarsella-30b005a0
Seattle, WA
David Scarsella
Director, Facilities, Occupational Health and Safety at NanoString Technologies, Inc.
https://www.linkedin.com/in/dan-dunn-1b12311b6
Bellevue, WA
Dan Dunn
Material Handler II at NanoString Technologies, Inc.
https://www.linkedin.com/in/zebulonw2
Seattle, WA
Zebulon Williams
University of Washington Graduate
https://www.linkedin.com/in/preston-ehrlich-b96569124
Seattle, WA
Preston Ehrlich
Nanostring Technologies Inc.
https://www.linkedin.com/in/halie-pitman-98381659
Greater Seattle Area
Halie Pitman
Manufacturing Research Associate at NanoString Technologies, Inc.
https://www.linkedin.com/in/conor-myhre-b88356178
Seattle, WA
Conor Myhre
Process Engineer at NanoString Technologies, Inc.
https://www.linkedin.com/in/hinesbrian
Edmonds, WA
Brian Hines
Research Associate IV at NanoString Technologies, Inc.
https://www.linkedin.com/in/brian-birditt-49827a1b8
Seattle, WA
brian birditt
Scientist at NanoString Technologies, Inc.
https://www.linkedin.com/in/james-rhydderch-0b553a5
Seattle, WA
James Rhydderch
Manager, CodeSet Production at NanoString Technologies
https://www.linkedin.com/in/forrest-beverly-23524b84
Greater Seattle Area
Forrest Beverly
Lab Supervisor of the Consumables Group at NanoString Technologies, Inc.
https://www.linkedin.com/in/andy-nam-259a3986
Greater Seattle Area
Andy Nam
Research Associate III at Nanostring
https://www.linkedin.com/in/gabejmorris
Kirkland, WA
Gabe Morris
Manufacturing Research Associate I at NanoString Technologies, Inc.
https://www.linkedin.com/in/edith-harris-8034b5ba
Seattle, WA
Edith Harris
Scientist:
Molecular & Cellular Biology  and Diagnostic Development
https://www.linkedin.com/in/brandon-skarset-4a17bb8b
Seattle, WA
Brandon Skarset
Manufacturing Research Associate IV at NanoString Technologies, Inc.
https://www.linkedin.com/in/matthew-hartman-939998133
Seattle, WA
Matthew Hartman
Manufacturing Research Associate III at Nanostring Technologies
https://www.linkedin.com/in/victoria-sangrey-hunter-66718711
Seattle, WA
Victoria Sangrey Hunter
Senior Executive Assistant, Sales & Marketing, Manage Meetings and Events
https://www.linkedin.com/in/faridabey
Seattle, WA
Farida Bey
Sr. Executive Assistant, Research and Development at NanoString Technologies
https://www.linkedin.com/in/megangrout
Greater Seattle Area
Megan (Grout) Vandenberg
Bioinformatics Scientist I at NanoString Technologies, Inc.
https://www.linkedin.com/in/matthew-orazem-202b8613
Seattle, WA
Matthew Orazem
Manager, CodeSet Manufacturing at NanoString Technologies, Inc.
https://www.linkedin.com/in/brian-filanoski-93215514
Vashon, WA
Brian Filanoski
Scientist at NanoString Technologies, Inc.
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Umoja is hiring! As we grow, we are looking for amazing people to join the team. If you love all things cells, check out this position:…
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I'm proud to announce that our paper describing the role of Set7 in the regulation of trained immunity was published in Cell Reports today. This…
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Greater Pittsburgh Region
Aaron M.
Close Protection Officer / Armed Security Officer
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Erie, CO
Aaron McClelland
Home Inspector Extraordinaire
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Virginia Beach, VA
Aaron McClelland
Transitioning from the Navy September 2022
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Ocala, FL
Aaron McClelland
Drilling Supervisor at Triple E contracting