aaron almeida
Manager: Manufacturing Process Optimization at Catalent Pharma Solutions
manager : manufacturing process optimization catalent pharma solution
pharmaceutical manufacturing leader core technical competency solid-phase chemistry , cell culture , chromatography filtration based purification method , bioconjugation chemistry structural/conformational analysis . experienced product development basic research vitro vivo testing , target optimization , process development , tox batch production technology transfer around biologic synthetic pharmaceutical agent . experienced creating leading client facing technical group focused around process data monitoring optimizing manufacturing operation .
Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Helped design, develop and roll out new group within pharmaceutical manufacturing operations. 
Technical project leadership around tech transfer, batch production records, process monitoring, process data trending, deviation investigation, CAPA, and optimization through process modeling with a focus on wholistic project analytics and client management

CI group leadership around standard work flow, standard process analytics strategies using large data sets, process automation and process modeling.
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Manager: Manufacturing Process Optimization
Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Group and lab management focused around tech transfer to GMP manufacturing. Optimize platform development strategies for rapid process identification and procedural optimizations for efficient tech transfer. 
SOP and BPR document drafting and review around GMP manufacturing and development activities.
Lab and inventory management around lean six sigma principles.
Manage programs and production runs with supply chain and procurement
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Group Leader/Supervisor: Downstream Process Development
Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Downstream Project Lead for programs in Process Development and Tech Transfer.
Manager of downstream support for programs in cell line development and upstream process development. 
Plan to Execute Pillar Lead for site rollout of the new inventory management system JD Edwards EnterpriseOne 9.1
Project Planner support for Process Development
and Supply Chain Lead for Continuous Improvement projects in Materials Management for PD and GMP MFG material supply
Middleton, WI
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Downstream Process Development Scientist
University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Course lecturer for ABT770: Product Development
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Adjunct Professor: Applied Biotechnology Program
University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Course Development for ABT 770: Product Development
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Honorary Fellow: Applied Biotechnology Program
Arrowhead Research Corp.
https://www.linkedin.com/company/arrowhead-pharmaceuticals
Main chemistry lead for RNA interference (RNAi) targeting ligand discovery research from initial concept and chemistry design, pharmacokinetic and biodistribution optimizations to early stage tox batch production.

Established and managed in-house peptide synthesis facility with 3 full time employees. The group regularly used a combination of solution and solid-phase chemistry to generate non-traditional peptides with modifications that enabled conjugation, improved in vivo stability and/or improved biodistribution. Peptide synthesis followed by HPLC purification and lyophilization was regularly used to isolate the peptide on the single milligram to multigram batch scale size.
Madison, Wisconsin Area
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Senior Scientist
University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
As protein structure is intrinsically linked to the conformation that it adopts in solution, my research worked to experimentally identify how fundamental chemical features contribute to the stability of the parallel beta-sheet conformation. I regularly carried out non-traditional peptide synthesis in combination with solution phase chemistry to generate peptide oligomers (e.g. 10-30) of alpha-amino acid residues which contain multiple diacids and diamines incorporated into the peptide backbone. 
	Traditional biophysical strategies (Circular Dichroism, Analytical Ultracentrifugation) were used in coordination with NMR analysis (chemical shift and nOe based) to determine the conformation of the peptides. The nOe's would then be incorporated into molecular dynamics simulations to calculate the conformation of the peptides in solution.
Chemistry Department
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Graduate Student
Ph.D. Chemistry
Under the supervision of Samuel H. Gellman
Organic Chemistry
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University of Wisconsin-Madison
https://www.linkedin.com/school/uwmadison/
Bachelors of Science
Under the supervision of Joanne D. Kehlbeck
Chemistry
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Union College
https://www.linkedin.com/school/union-college/
HIF2α targeted RNAi therapeutic inhibits clear cell renal cell carcinoma
Molecular cancer therapeutics
http://mct.aacrjournals.org/content/early/2017/10/27/1535-7163.MCT-17-0471
Proteinogenic amino acid residues that promote β-sheet secondary structure are hydrophobic (e.g., Ile or Val) or only moderately polar (e.g., Thr). The design of peptides intended to display β-sheet secondary structure in water typically requires one set of residues to ensure conformational stability and an orthogonal set, with charged side chains, to ensure aqueous solubility and discourage self-association. Here we describe new amino acids that manifest substantial β-sheet propensity, by virtue of β-branching, and also bear an ionizable group in the side chain.
New Charge-bearing Amino Acid Residues That Promote Beta-Sheet Secondary Structure
J. Am. Chem. Soc
http://pubs.acs.org/doi/abs/10.1021/ja510265e
We report the asymmetric synthesis of the γ-amino acid (1R,2R)-2-aminomethyl-1-cyclopentane carboxylic acid (AMCP) and an evaluation of this residue’s potential to promote secondary structure in α/γ-peptides. Simulated annealing calculations using NMR-derived distance restraints obtained for α/γ-peptides in chloroform reveal that AMCP-containing oligomers are conformationally flexible. However, additional evidence suggests that an internally hydrogen-bonded helical conformation is partially populated in solution. From these data, we propose characteristic NOE patterns for the formation of the α/γ-peptide 12/10-helix and discuss the apparent conformational frustration of AMCP-containing oligomers
Evaluation of a Cyclopentane-Based γ-Amino Acid for the Ability to Promote α/γ-Peptide Secondary Structure
Journal of Organic Chemistry
http://pubs.acs.org/doi/abs/10.1021/jo401501g?prevSearch=%255BContrib%253A%2Bgiuliano%252C%2Bm.w.%255D&searchHistoryKey=
Authors: Woys, A., Almeida A.M., Wang L., Chiu C-C, McGovern, M.P. de Pablo J.J., Skinner, J.L., Gellman, S.H., Zanni, M.T.
Parallel β-Sheet Vibrational Couplings Revealed by 2D IR Spectroscopy of an Isotopically Labeled Macrocycle: Quantitative Benchmark for the Interpretation of Amyloid and Protein Infrared Spectra.
J. Am. Chem. Soc
http://pubs.acs.org/doi/abs/10.1021/ja3074962
Disulfide bonds between Cys residues in adjacent strands of parallel β-sheets are rare among proteins, which suggests that parallel β-sheet structure is not stabilized by such disulfide cross-links. We report experimental results that show, surprisingly, that an interstrand disulfide bond can stabilize parallel β-sheets formed by an autonomously folding peptide in aqueous solution. NMR analysis reveals that parallel β-sheet structure is terminated beyond the disulfide bond, which causes deviation from the extended backbone conformation at one of the Cys residues.
Parallel β-Sheet Secondary Structure Is Stabilized and Terminated by Interstrand Disulfide Cross-Linking
J. Am. Chem. Soc.
http://pubs.acs.org/doi/abs/10.1021/ja208856c
Authors: Dr. Felix Friere (+), Aaron M. Almeida(+), John D. Fisk, Jay D. Steinkruger, Prof. Samuel H. Gellman 
These Authors contributed equally

Molecular design can be used to investigate whether the parallel-β-sheet secondary structure is subject to length-dependent cooperative stabilization along the strand direction. Extending strands by adding high-propensity residues leads to a steady increase in parallel-β-sheet stability, but inclusion of low-propensity residues can negate this stabilization.
Impact of Strand Length on the Stability of Parallel-β-Sheet Secondary Structure
Wiley/ Angewandte Chemie
http://onlinelibrary.wiley.com/doi/10.1002/anie.201102986/abstract
Authors: Guo L, Almeida AM, Zhang W, Reidenbach AG, Choi SH, Guzei IA, Gellman SH.

We report the first high-resolution structural data for the beta/gamma-peptide 13-helix (i,i+3 C=O...H-N H-bonds), a secondary structure that is formed by oligomers with a 1:1 alternation of beta- and gamma-amino acid residues. Our characterization includes both crystallographic and 2D NMR data. Previous studies suggested that beta/gamma-peptides constructed from conformationally flexible residues adopt a different helical secondary structure in solution. Our design features preorganized beta- and gamma-residues, which strongly promote 13-helical folding by the 1:1 beta/gamma backbone.
Helix formation in preorganized beta/gamma-peptide foldamers: hydrogen-bond analogy to the alpha-helix without alpha-amino acid residues.
J. Am. Chem. Soc.
http://pubs.acs.org/doi/abs/10.1021/ja103233a
Authors : Li Guo, Yonggui Chi, Aaron M. Almeida, Ilia A. Guzei, Brian K. Parker and Samuel H. Gellman

A highly stereoselective synthesis of novel cyclically constrained γ-amino acid residues is presented. The key step involves organocatalytic Michael addition of an aldehyde to 1-nitrocyclohexene. After aldehyde reduction, this approach provides optically active β-substituted δ-nitro alcohols (96−99% ee), which can be converted to γ-amino acid residues with a variety of substituents at the α position. We have used these new building blocks to prepare α/γ-peptide foldamers that adopt a specific helical conformation in solution and in the solid state.
Stereospecific synthesis of conformationally constrained gamma-amino acids: new foldamer building blocks that support helical secondary structure,
J. Am. Chem. Soc.
http://pubs.acs.org/doi/abs/10.1021/ja907233q
A γ-Amino Acid that Favors 12/10-Helical Secondary Structure in α/γ-Peptides
http://pubs.acs.org/doi/abs/10.1021/ja5076585
Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification
https://aiche.onlinelibrary.wiley.com/doi/10.1002/btpr.3227
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Greater Madison Area
Kimberly S.
Research Specialist at AgraFilms
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Madison, WI
Lucas King
Supervisor, cGMP Downstream/mRNA Therapeutic Biomanufacturing  at Catalent Pharma Solutions
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Greater Madison Area
Ethan Grant
Senior Scientist I at Catalent Pharma Solutions
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Greater Madison Area
Kevin Jose
Sr. Group Leader at Catalent
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Raleigh, NC
Connor Rupke
--
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Los Angeles Metropolitan Area
Kevin Vergenz
Los Angeles, CA
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Madison, WI
Bhaumik Vyas
Senior Process Engineer at Catalent Pharma Solutions
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Watertown, MA
Eric York
Head of Data Visualization @ Wayfair
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Madison, WI
Jennifer Finta
Process Engineer at Catalent Pharma Solutions
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Denver Metropolitan Area
Shannon M. Flahive
Venture Capital Investor | Business Strategist
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Boston, MA
Radha Patel
VP, Retail @ Whoop
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Seguin, TX
Ronald Zuniga
Supervisor Downstream Biomanufacturing, Fujifilm. College Station, Texas at FUJIFILM Diosynth Biotechnologies
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Greater Madison Area
Darren Thiel
Quality Assurance Specialist at Catalent Pharma Solutions
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Madison, WI
Katie Berth
Manager, flex MS&T at Catalent Pharma Solutions
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Greater Madison Area
Joshua Maurer
Quality Assurance Supervisor at Catalent Pharma Solutions
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Milwaukee, WI
William Dunmire
Recreations Director at Valley Lo Club
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Madison, WI
Emily Schirmer
Senior Director Process Development at Catalent Pharma Solutions
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Berkeley, CA
Jitendra Mishra, PhD, MBA
Associate Director
https://www.linkedin.com/in/guohua-liang-90a2a7b
Acton, MA
Guohua Liang
Senior Manager, API Manufacturing and Process Development at Avedro, Inc.
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Solicitor
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Bengaluru
Aaron Almeida
SAP Supply Chain Senior Consultant at Deloitte US-India | IBM Alumnus | NITK Alumnus
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United Arab Emirates
Aaron Almeida
Regional Data Analyst at TikTok
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Mumbai
Aaron Almeida
Associate Consultant at Oracle Financial Services Software Limited
Wisconsin Alumni (Official)
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Oligonucleotide and Peptide Development Professionals
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Union College Alumni Group
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Samuel H. Gellman Group Alumni
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Midwest Life Science Network (LSN)
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University of Wisconsin–Madison
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TIDES (Peptides & Oligonucleotides) Community
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Organic Chemists in Drug Discovery
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Peptide Synthesis
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