Aline Mello
Scientist I at Cepheid
Scientist I at Cepheid
Cepheid
https://www.linkedin.com/company/cepheid
Sunnyvale, California, United States
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Scientist I
Cepheid
https://www.linkedin.com/company/cepheid
Sunnyvale, California, United States
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Scientific Researcher
University of Pittsburgh
https://www.linkedin.com/company/university-of-pittsburgh
Pittsburgh, Pennsylvania, United States
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Posdoctoral Researcher
University of California, Davis
https://www.linkedin.com/company/uc-davis
Assistant in classes, lead discussion sessions and test correction Disciplines: Cell Biology (3 quarters), Introduction to Human Heredity (2 quarters), Genes and Genetics (1 quarter)
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Teaching Assistant
NIH Superfund Research Program at UCDavis
Trainee of NIH Superfund Research Program at UCDavis Project 5 : Endoplasmic Reticulum Stress Within the goals of the UC Davis Superfund Center, this project investigate new mechanistic insights into the effects of chronic exposure of Superfund (SF) chemicals on endoplasmic reticulum (ER) stress, which leads to fibrosis and organ damage. Recent and exciting data demonstrate that exposure to xenobiotics perturbs the levels of P450-generated lipid mediators, which directly affect the pathological development of ER stress. Thus, this project directly test the novel hypothesis that plasma lipid mediators may represent a surrogate biomarker for ER stress following chronic xenobiotic exposure, as well as developing a new cell-based bioassay for quantitation of pro-ER stress environmental toxicants in field samples.
NIH Superfund Research Program Trainee
University of California, Davis
Dr. Fawaz Haj’s Lab – Nutrition Department - UCDavis - Feb 2015 – Dez 2019
Studies of the molecular basis of metabolic diseases with a focus on the liver contribution to the systemic homeostasis and physiology. Using small animal models, biochemistry, cellular and molecular biology techniques.

Project 1: Soluble epoxide hydrolase is a potential therapeutic target for non-alcoholic steatohepatitis

Project 2: Soluble epoxide hydrolase is a potential therapeutic target for alcoholic steatohepatitis

Project 3: Soluble epoxide hydrolase role in acute liver injury
Davis, CA
Graduate Student Researcher
Doctor of Philosophy - PhD
Research studying the role of the enzyme sEH and its oxilipids on the molecular mechanism of metabolic diseases as Non-Alcoholic Steatohepatitis and Alcoholic Steatohepatitis. Using small animal models, biochemistry, cellular and molecular biology techniques.
Biochemistry, Molecular, Cellular and Development Biology
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University of California, Davis
https://www.linkedin.com/school/uc-davis/
Master of Sciences in Structural and Cellular Biology
Investigation of protein partners for the kinase SRPK2 in the regulation of alternative splicing using yeast-two-hybrid technique, microbiology culture, plasmid cloning and genomic library.
Molecular and Cellular Biology
Federal University of Viçosa
Biological Sciences Bachelor
Molecular Biology and Biochemistry
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Federal University of Viçosa
https://www.linkedin.com/school/universidade-federal-de-vi-osa/
Background & aims: We recently showed that alcoholic hepatitis (AH) is characterized by dedifferentiation of hepatocytes and loss of mature functions. Glucose metabolism is tightly regulated in healthy hepatocytes. We hypothesize that AH may lead to metabolic reprogramming of the liver, including dysregulation of glucose metabolism.

Methods: We performed integrated metabolomic and transcriptomic analyses of liver tissue from patients with AH or alcoholic cirrhosis or normal liver tissue from hepatic resection. Focused analyses of chromatin immunoprecipitation coupled to DNA sequencing was performed. Functional in vitro studies were performed in primary rat and human hepatocytes and HepG2 cells.

Results: Patients with AH exhibited specific changes in the levels of intermediates of glycolysis/gluconeogenesis, the tricarboxylic acid cycle, and monosaccharide and disaccharide metabolism. Integrated analysis of the transcriptome and metabolome showed the used of alternate energetic pathways, metabolite sinks and bottlenecks, and dysregulated glucose storage in patients with AH. Among genes involved in glucose metabolism, hexokinase domain containing 1 (HKDC1) was identified as the most up-regulated kinase in patients with AH. Histone active promoter and enhancer markers were increased in the HKDC1 genomic region. High HKDC1 levels were associated with the development of acute kidney injury and decreased survival. Increased HKDC1 activity contributed to the accumulation of glucose-6-P and glycogen in primary rat hepatocytes.

Conclusions: Altered metabolite levels and messenger RNA expression of metabolic enzymes suggest the existence of extensive reprogramming of glucose metabolism in AH. Increased HKDC1 expression may contribute to dysregulated glucose metabolism and represents a novel biomarker and therapeutic target for AH.
Integrated Multi-Omics Analysis Reveals Glucose Metabolic Reprogramming and Identifies A Novel Hexokinase In Alcoholic Hepatitis
Gastroenterology
https://www.sciencedirect.com/science/article/abs/pii/S0016508520355426?via%3Dihub
Background & Aims
Alcohol-associated liver disease (ALD) is a significant cause of liver-related morbidity and mortality worldwide and with limited therapies. Soluble epoxide hydrolase (sEH; Ephx2) is a largely cytosolic enzyme that is highly expressed in the liver and is implicated in hepatic function, but its role in ALD is mostly unexplored.

Methods
To decipher the role of hepatic sEH in ALD, we generated mice with liver-specific sEH disruption (Alb-Cre; Ephx2fl/fl). Alb-Cre; Ephx2fl/fl and control (Ephx2fl/fl) mice were subjected to an ethanol challenge using the chronic plus binge model of ALD and hepatic injury, inflammation, and steatosis were evaluated under pair-fed and ethanol-fed states. In addition, we investigated the capacity of pharmacologic inhibition of sEH in the chronic plus binge mouse model.

Results
We observed an increase of hepatic sEH in mice upon ethanol consumption, suggesting that dysregulated hepatic sEH expression might be involved in ALD. Alb-Cre; Ephx2fl/fl mice presented efficient deletion of hepatic sEH with corresponding attenuation in sEH activity and alteration in the lipid epoxide/diol ratio. Consistently, hepatic sEH deficiency ameliorated ethanol-induced hepatic injury, inflammation, and steatosis. In addition, targeted metabolomics identified lipid mediators that were impacted significantly by hepatic sEH deficiency. Moreover, hepatic sEH deficiency was associated with a significant attenuation of ethanol-induced hepatic endoplasmic reticulum and oxidative stress. Notably, pharmacologic inhibition of sEH recapitulated the effects of hepatic sEH deficiency and abrogated injury, inflammation, and steatosis caused by ethanol feeding.

Conclusions
These findings elucidated a role for sEH in ALD and validated a pharmacologic inhibitor of this enzyme in a preclinical mouse model as a potential therapeutic approach.
Soluble epoxide hydrolase hepatic deficiency ameliorates alcohol-associated liver disease.
CMGH
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851189/
Alcoholic liver disease (ALD) is a major health problem and a significant cause of liver-related death. Currently, the mainstay for ALD therapy is alcohol abstinence highlighting the need to develop pharmacotherapeutic approaches. Protein-tyrosine phosphatase 1B (PTP1B) is an established regulator of hepatic functions, but its role in ALD is mostly unexplored. In this study, we used mice with liver-specific PTP1B disruption as well as pharmacological inhibition to investigate the in vivo function of this phosphatase in ALD. We report upregulation of hepatic PTP1B in the chronic plus binge mouse model and, importantly, in liver biopsies of alcoholic hepatitis patients. Also, mice with hepatic PTP1B disruption attenuated ethanol-induced injury, inflammation, and steatosis compared with ethanol-fed control animals. Moreover, PTP1B deficiency was associated with decreased ethanol-induced oxidative stress in vivo and ex vivo. Further, pharmacological modulation of oxidative balance in hepatocytes identified diminished oxidative stress as a contributor to the salutary effects of PTP1B deficiency. Notably, PTP1B pharmacological inhibition elicited beneficial effects and mitigated hepatic injury, inflammation, and steatosis caused by ethanol feeding. In summary, these findings causally link hepatic PTP1B and ALD and define a potential therapeutic target for the management of this disease.
Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in mice.
Redox Biol.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408361/
Objective
Diabetic nephropathy is one of the most devastating complications of diabetes, and growing evidence implicates podocyte dysfunction in disease pathogenesis. The objective of this study was to investigate the contribution of protein tyrosine phosphatase 1B (PTP1B) in podocytes to hyperglycemia-induced renal injury.

Methods
To determine the in vivo function of PTP1B in podocytes we generated mice with podocyte-specific PTP1B disruption (hereafter termed pod-PTP1B KO). Kidney functions were determined in control and pod-PTP1B KO mice under normoglycemia and high-fat diet (HFD)- and streptozotocin (STZ)-induced hyperglycemia.

Results
PTP1B expression increased in murine kidneys following HFD and STZ challenges. Under normoglycemia control and pod-PTP1B KO mice exhibited comparable renal functions. However, podocyte PTP1B disruption attenuated hyperglycemia-induced albuminuria and renal injury and preserved glucose control. Also, podocyte PTP1B disruption was accompanied with improved renal insulin signaling and enhanced autophagy with decreased inflammation and fibrosis. Moreover, the beneficial effects of podocyte PTP1B disruption in vivo were recapitulated in E11 murine podocytes with lentiviral-mediated PTP1B knockdown. Reconstitution of PTP1B in knockdown podocytes reversed the enhanced insulin signaling and autophagy suggesting that they were likely a consequence of PTP1B deficiency. Further, pharmacological attenuation of autophagy in PTP1B knockdown podocytes mitigated the protective effects of PTP1B deficiency.

Conclusions
These findings demonstrate that podocyte PTP1B deficiency attenuates hyperglycemia-induced renal damage and suggest that PTP1B may present a therapeutic target in renal injury.
Protein tyrosine phosphatase 1B deficiency in podocytes mitigates hyperglycemia-induced renal injury.
Metabolism.
https://www.sciencedirect.com/science/article/pii/S0026049517302068?via%3Dihub
Nuptial flight is a critical phase in the reproduction of most ant species. Here, we describe the nuptial flight and mating behaviour of Acromyrmex ameliae, a leaf-cutting ant social parasite. We observed a nuptial flight of this parasite in a parasitized colony of Acromyrmex subterraneus subterraneus – known as a host of the social parasite in nature – and we registered the initial and final times of copula as well as the time the male dispended attempting to mate. Mating flight occurred after the beginning of the light phase in the laboratory. Agitation of host workers preceded the nuptial flight, without aggression between host workers and sexuals. Alates were attracted to the light bulbs of the room and formed couples that dropped to the ground immediately after their formation. Copula lasted on the average 1.6 ± 0.6 min. During mating attempts, the male put his forelegs on the female's abdomen while she remained walking on the ground. Copula normally occurred when the female stopped or slowed her movement. No attempt at remating was observed. Dealate females were offered to colonies of Acromyrmex subterraneus molestans, a taxon not known as a host of the social parasite in nature, and were promptly accepted by these colonies. The sex ratio value (males:females) was 1:1.83, another factor suggesting that females mate with only one male.
A laboratory observation of nuptial flight and mating behaviour of the parasite ant Acromyrmex ameliae (Hymenoptera: Formicidae).
Italian Journal of Zoology
https://www.tandfonline.com/doi/full/10.1080/11250003.2010.490567
Funding in recognition of the impact of my research for traveling and presenting my research in the 2018 Superfund Research Program Annual Meeting.
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Carpenter Travel Award
Funding in recognition of the impact of my research during the summer of 2018
NIH
NIEHS/UC Davis Superfund Research Summer 2018 GSR Stipend
Program for Doctoral candidate training abroad
September 2014 – August 2018
CAPES/Brazil
Scholarship Science without Borders, SwB
Scholarship associated to Master’s Degree
September 2012 - July 2014
CAPES/Brazil
Master’s Degree Program CAPES/Brazil
Young Scientist program bachelor’s level
Scholarship associated with the project "Investigation of protein partners for the kinase SRPK2 in the regulation of alternative splicing"
August 2011 - July 2012
CAPES/Brazil
Scholarship PIBIC/CAPES
Scholarship to perform activities related to teaching, research and extension.
August 2008 – July 2011
CAPES/Brazil
Scholarship PET/SESu
https://www.linkedin.com/in/nichole-lewis5bb1331ab
Davis, CA
Nichole Lewis
Cell and Molecular Biology Researcher | Science Educator | PhD Candidate at University of California Davis
https://www.linkedin.com/in/lukebogart
Cambridge, MA
Luke Bogart, Ph.D.
Principal Scientist, Neuroscience at Novartis Institutes for BioMedical Research
https://br.linkedin.com/in/suellen-pereira
Uberlândia, MG
Suéllen Pereira , PMP®
Technical Program Management
https://dk.linkedin.com/in/gitte-holmen-olofsson-5379156a
Copenhagen
Gitte Holmen Olofsson
Postdoc hos CCIT - Center for Cancer Immune Therapy
https://www.linkedin.com/in/ramon-bataller-a7633344
Pittsburgh, PA
Ramon Bataller
Chief of Hepatology at University of Pittsburgh
https://www.linkedin.com/in/alison-ke-5510b5b6
Berkeley, CA
Alison Ke
Ph.D. Student at University of California, Davis
https://www.linkedin.com/in/terry-wheatley-79272715
Napa, CA
Terry Wheatley
President at Vintage Wine Estates
https://www.linkedin.com/in/cindy-pickett
Davis, CA
Cynthia Pickett, PhD
Associate Provost for Diversity, Equity, and Inclusion at DePaul University, Social Psychologist, DEI Thought Leader
https://www.linkedin.com/in/taryn-west91
Hayward, CA
Taryn W.
Program Operations Manager at Boys & Girls Clubs of the Peninsula
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San Francisco, CA
Jeff Cole
Full-time chem and bio teacher. Part-time nursing work wanted
https://br.linkedin.com/in/mireli-m%C3%BCller-silveira-43198914a
Lençóis Paulista, SP
Mireli Müller Silveira
Bióloga, Mestre em Engenharia Ambiental
https://www.linkedin.com/in/paul-maxson-6b056166
Redwood City, CA
Paul Maxson
I'll find you!
https://www.linkedin.com/in/jp-zarco-331a11174
San Francisco Bay Area
Jp Zarco
50/50 Raffle seller at Golden State Warriors
https://www.linkedin.com/in/juliaspringhuang
Davis, CA
Julia Huang
Student at UC Davis
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San Diego, CA
Anika Ayson
Project Manager | Digital Marketing Analyst
https://www.linkedin.com/in/vanessacodilla
Los Angeles Metropolitan Area
Vanessa Codilla
Communications Specialist at UCLA IRLE | M.S. in Digital Media Management at USC Annenberg, '23
https://br.linkedin.com/in/guilherme-borges-weege-71734a108
Canguçu, RS
Guilherme Borges Weege
Doutorando | Parasitologia e Imunologia Aplicada
https://www.linkedin.com/in/pjgallagher2010
San Francisco Bay Area
Patrick Gallagher
Engineering Leadership Community Builder
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São Paulo, Brazil
Yasmine Alves Menegon
Biotechnologist - 
Bioenergy Ph.D. Candidate
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Check out this nice review on Moderate Alcoholic Hepatitis from my lab. One more for the team!You can download it for free for the next 50 days…
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Check out the most recent paper from my lab in glucose metabolism and Alcoholic Hepatitis. You can download it for free for the next 50 days using…
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Quem aí também gosta de encontrar serpentes na trilha? #vidasilvestre
https://br.linkedin.com/in/aline-mello-4461b31b0
Rio de Janeiro, Brazil
ALINE MELLO
CEO na PleneCare Soluções em Saúde, Voluntário na Associação fazendo o bem sem olhar a quem
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Araraquara, SP
Aline Mello
SAP Basis Security | License Auditor SAP | Solution Manager
https://www.linkedin.com/in/alinemello
San Francisco Bay Area
Lillie Mello
Learning & Development • Industrial-Organizational Psychologist • People & Culture • Program Management
https://br.linkedin.com/in/aline-mello-6081ba26
Brazil
Aline Mello
Senior Manager at The HEINEKEN Company
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Rio de Janeiro, RJ
Aline Mello
QSMS & Compliance / 
Auditora Líder do SGI/
Assistente Técnica Pericial Ambiental & Insalubridade e Periculosidade
Global NASH Network by Nathan Nagel
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