Aanchal Mehrotra
NGS Quality Scientist at Adaptive Biotechnologies Corp.
NGS Quality Scientist at Adaptive Biotechnologies Corp.
A research scientist with more than 15 years of hands on BSL-2 and regulated  experience in biomedical research involving genome editing, Assay development, Cell, and tissue culture, primary cell culture, cell line generation, transfections, transductions, molecular cloning,  knockdown and overexpression of specific genes of particular genes in vitro assay development, phenotypic and target-based screening. 

• Experience with FFPE tissue, nucleic acid extraction, quantification, and quality analysis, library preparation, Next-generation sequencing (Illumina- MiSeq, NextSeq550/500),  and tissue culture (BSL-2) both with primary and stable cell lines. 

• Immunoassay development including ELISA, Luminex, MSD, and Quanterix platforms. 

• Experience developing, implementing, and supporting high complexity assays in a regulated environment including writing standard operating procedures, validation protocols, and data reports.

• Extensive experience with presentations and technical writing, problem-solving capability, able to work with deadlines.
Adaptive Biotechnologies Corp.
https://www.linkedin.com/company/adaptive-biotechnologies-corp-
Primary Responsibilities
➢ Analyze and troubleshoot data from the QC processing of NGS reagents and consumables used in Adaptive Biotechnology’s regulated, high complexity, clinical molecular testing facility(CLIA lab).
➢ Plan, conduct, analyze and report non-routine investigation of NGS quality failures and trends in a timely manner, in coordination with QC, Laboratory Operations Improvement, and Manufacturing teams.
➢ Provide input to and be familiar with all procedures and processes relevant to QC of reagents/consumables (NGS, extraction, Q-PCR, PCR) and kits
➢ Serve as a Subject matter expert (SME) and communication liaison to multidisciplinary teams.
➢ Coordinate with Product Development and molecular Research and innovation teams.
➢ Ensure proper documentation of investigations, analyses, and procedures performed.
➢ Coordinate with and assist process improvements team.
➢ Provide input and review regarding various analytical issues to other teams as needed.
➢ Complete Nonconformance, Deviation, or CA/PA documents as needed/assigned.
➢ Assist in root cause analyses of issues affecting production processes and reagents.
➢ Provide clear and effective communication to staff engaged in QC testing.
➢ Escalate repeat QC procedure failures or concerns as appropriate.
➢ Review Quality Control data for accuracy, completeness, and compliance with effective SOPs.
Seattle, Washington, United States
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NGS Quality Scientist
Seattle AWIS
https://www.linkedin.com/company/seattle-awis
Association for women in Science, Seattle chapter
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AWIS mentor
University of Washington - School of Medicine
https://www.linkedin.com/company/uwsomwwami
Research responsibilities: working on  cutting edge technologies involving gene editing and next generation sequencing in  a CLIA lab.  Specifically involved in Saturation Genome Editing experiments which is a survival based cellular assay. It involves characterizing thousands of variants of uncertain significance with ease by introducing a library of all possible SNVs for a particular locus as a HDR template along with a CRISPR-cas9 guide targeting the region in question. 
Expertise in handling large data sets with ease. Performing data analysis and interpretation using R, python, and statistical software such as graph pad prism. Comfortable using Bash, a unix shell and command language on the terminal and perform data handling, management and analysis with ease.
Few more focused responsibilities:
•	Design of  NGS based Experiments (DOE) and perform validation studies.
•	Measure and improve assay robustness by band-guarding experimental parameters.
•	Sample processing, acquiring data, performing extensive data analysis, generating detailed reports.
•	 Lead data discussion in collaborator  meetings and build research collaborations. 
•	Project administration: Agendas, minutes, communication, and document management.
•	Project coordination: Critical path analysis, action item and task management and follow up, identifying and clarifying task dependencies.
•	Project Management: Team facilitation, problem solving mediation, critical path and parameter analysis.
•	Writing and revising documents: Standard operating procedures (SOPs), batch records etc.
Greater Seattle Area
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Scientist-III,   Dept of Genome Science
NanoString Technologies, Inc.
https://www.linkedin.com/company/nanostring-technologies
Primary Responsibilities: 
•Design  of  Experiments  (DOE)  and  perform diagnostic  validation  assays  on  breast cancer/lymphoma  FFPE  samples  from  various  collaborators  as  well  as  Pharma companies all over the world
.•Measure and improve assay robustness by band-guarding experimental parameters.
•Sample  processing,  acquiring  data,  performing  extensive  data  analysis,  generating detailed  reports  containing  the  gene  expression  data  from  the  various  immune-oncology  as  well  as  Breast  Cancer  gene  expression  panels  (770  genes  in  each panel).
•Lead  data  discussion  in  collaborator  and  investigational  use  only  (IUO)  clinical meetings and build research collaborations for NanoString. 
•Project    administration:    Agendas,    minutes,    communication,    and   document management.
•Project coordination: Critical path analysis, action item and task management and follow up, identifying and clarifying task dependencies.
•Project Management:Team facilitation, problem solving mediation, critical path and parameter analysis.
•Writing  and  revising  documents:  Standard  operating  procedures  (SOPs),  batch records etc.
Seattle, Washington, United States
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Scientist-II
University of Washington - School of Medicine
https://www.linkedin.com/company/uwsomwwami
In this role I was involved in comprehensive research towards biomarker discovery and development essential in the diagnosis and staging of neurodegenerative diseases and for the evaluation of potential disease modifying agents. I was also involved in handling huge cohorts of clinical samples (CSF and plasma) and performed a proteomic profiling via Mass Spectrometry as well as development of new immunoassays on various platforms such as ELISA, Luminex and Quanterix- SIMOA assays.
Greater Seattle Area
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Scientist-II
University of Washington
https://www.linkedin.com/company/university-of-washington
At UW I was working on understanding the  mechanisms which are critical for development of better models  i) to assess drug disposition in fetuses, children & Adults and ii) to accurately predict interindividual variability in drug pharmacokinetics (PK) and pharmacodynamics (PD) mediated by factors affecting these mechanisms.
Greater Seattle Area
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Senior Postdoctoral Fellow
UNIVERSITY OF TOLEDO MEDICAL CENTER
https://www.linkedin.com/company/university-of-toledo-medical-center
During my postdoc, I have worked on SWI/SNF chromatin remodeling complexes and have tried to find out the relationship, of this very important chromatin remodeling complex in the deadliest form of skin cancer i.e. Melanoma. For this, we concentrated on one very important BRAF mutation i.e. BRAF V600E which is responsible for promoting   epigenetic changes in melanomas. Our hypothesis was that BRAF (V600E) alters SWI/SNF function to promote the epigenetic changes that occur in melanoma.
 I have also worked on elucidating mechanisms by which microRNAs can regulate SWI/SNF chromatin remodeling enzymes in being responsible for the gene expression changes occurring under pathological hypertrophic conditions. For this we were using both in-vitro, primary cardiomyocytes from 1-2 day old rats and in-vivo models such as the Dahl/Rapp salt sensitive and resistant rats.
Ohio, USA
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Postdoctoral Research Associate
Postgraduate Institute of Medical Education and Research
https://www.linkedin.com/company/postgraduate-institute-of-medical-education-and-research
This is an academic Faculty position responsible for the course work of M.S. Medical Biotechnology students at PGIMER, Chandigarh, India.
Chandigarh Area, India
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Junior Demonstrator/ Acting instructor
Doctor of Philosophy - PhD
Molecular Medicine and Biotechnology
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Postgraduate Institute of Medical Education and Research
https://www.linkedin.com/school/postgraduate-institute-of-medical-education-and-research/
Master of Science (M.S.)
Medical Biotechnology
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Postgraduate Institute of Medical Education and Research
https://www.linkedin.com/school/postgraduate-institute-of-medical-education-and-research/
Bachelor of Science (B.S.) Honours Biotechnology
Biotechnology
Banasthali Vidhyapith, Rajasthan, India
Healthcare Businesswomen's association (HBA)
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Women in Bio (WIB)
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Faculty of 1000
member
American Society for Pharmacological and Experimental Therapeutics(ASPET)
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National Postdoctoral Association (NPA)
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Women in Cancer Research (WICR)
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European association of Cancer Research (EACR)
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The American Society for Biochemistry and Molecular Biology (ASBMB)
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American Heart Association (AHA)
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Bromodomain and extra-terminal domain (BET) proteins regulate melanocyte differentiation.
Epigenetics and Chromatin
https://www.ncbi.nlm.nih.gov/pubmed/32151278
Impact of Pre-Analytical Differences on Biomarkers in the ADNI and PPMI Studies: Implications in the Era of Classifying Disease Based on Biomarkers.
Journal of Alzheimers Disease
https://www.ncbi.nlm.nih.gov/pubmed/30958379
Age- and genotype-dependent variability in the protein abundance and activity of six major uridine diphosphate-glucuronosyltransferases in human liver.
Clinical Pharmacology and  Therapeutics
Anti-diabetic vanadyl complexes reduced Alzheimer's disease pathology independent of amyloid plaque deposition.
Sci China Life Sci
Hepatic Abundance and Activity of Androgen and Drug Metabolizing Enzyme, UGT2B17, are Associated with Genotype, Age, and Sex.
Drug Metab Dispos
https://www.ncbi.nlm.nih.gov/pubmed/29602798
BRG1 interacts with SOX10 to establish the melanocyte lineage and to promote differentiation.
Nucleic Acids Res.
https://www.ncbi.nlm.nih.gov/pubmed/28431046
Age-Dependent Absolute Abundance of Hepatic Carboxylesterases (CES1 and CES2) by LC-MS/MS Proteomics: Application to PBPK Modeling of Oseltamivir In Vivo Pharmacokinetics in Infants.
Drug Metab Dispos.
The Promises of Quantitative Proteomics in Precision Medicine.
J Pharm Sci.
Modulation of BRAHMA expression by the mitogen-activated protein kinase / extracellular signal regulated kinase (ERK1/2) pathway is associated with changes in melanoma proliferation
Archives of Biochemistry and Biophysics
http://www.ncbi.nlm.nih.gov/pubmed?cmd=historysearch&querykey=7
It got published as a cover article
SWI/SNF Chromatin Remodeling Enzymes in Melanocyte Differentiation and Melanoma
Critical Reviews in Eukaryotic Gene Expression.
http://www.ncbi.nlm.nih.gov/pubmed/24940768
SWI/SNF enzymes promote SOX10- mediated activation of myelin gene expression
Plos one
http://www.ncbi.nlm.nih.gov/pubmed/23874858
Enhanced SWI/SNF enzyme recruitment and chromatin remodeling on fetal cardiac gene promoters is associated with cardiac hypertrophy in a genetic rat model of hypertension.
journal of cellular physiology
http://www.ncbi.nlm.nih.gov/pubmed/23702776
•	The Involvement of SWI/SNF chromatin remodeling enzymes in Transcriptional Regulation of pathological Cardiac Hypertrophy.
FASEB Journal
LXR-α selectively reprogrammes cancer cells to enter into apoptosis.
Mar 2011
http://link.springer.com/article/10.1007%2Fs11010-010-0659-3
Hyperglycemic dependent LXR-alpha Gene Regulation within Blood Mononuclear Cells of CHD Patients
2011
http://www.omicsonline.org/2155-9880/2155-9880-2-117.php
E2F-1 RNomics is critical for reprogramming of cancer cells to quiescent state.
Aug 2010
http://onlinelibrary.wiley.com/doi/10.1002/ijc.25109/abstract;jsessionid=81FE5A22DD3AFE5F1F8118BF9850F0EA.d04t02
Functional characterization of AATF transcriptome in human leukemic cells.
Mar 2007
http://link.springer.com/article/10.1007%2Fs11010-006-9317-1
a review article
RNome: Macro-role in Molecular Medicine
2007
Manuscript in preparation
SWI/SNF and microRNA connection in transcriptional regulation of pathological cardiac hypertrophy
American society for Pharmacology and Experimental Therapeutics (ASPET), Experimental Biology meeting, San Diego
Best presentation and abstract award
Department of Biotechnology (DBT), Govt. Of India
“International Travel Award”
• Passed the prestigious national level Fellowship of Indian council of Medical Research (ICMR), India, during the course of PhD degree [ 2006-2011].
Indian council of MEdical Education and Research, India
National Level Fellowship
• Recipient of Major General Amir Chand Silver medal for the paper entitled: "E2F-1 RNomics is critical for reprogramming of cancer cells to quiescent state." published in the prestigious international journal of cancer.
PostGraduate Institute of MEdical Education and Research, Chandigarh, India
Major General Amir Chand silver medal
Seattle AWIS
https://www.linkedin.com/company/seattle-awis
I organize monthly AWIS meetings to discuss and mentor a group of extremely talented women scientists in Seattle area about preparations for future dream jobs, life lessons and and various career choices available after grad school.
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Mentor
Science and Technology
YMCA of Greater Seattle
https://www.linkedin.com/company/ymca-of-greater-seattle
Educating young minds about the importance of science and technology
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Guest Speaker
Health
American Heart Association
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Volunteer
Project Management Institute
AUSedg7dUNN6G4m7N9VOyTHbXVgx
Six Sigma: Black Belt
Project Management Institute
AWXkf9NIOn7Z5JVehQfPLqUYsiL7
Agile Project Leadership
The Leadership Edge
From the Laboratory to Leadership
National Association of State Boards of Accountancy (NASBA)
AQBDRY5JUGTrBObplKD5csyjuSYh
Setting Team and Employee Goals Using SMART Methodology
Project Management Institute
AbzpmGdUcW41Njcelz040M2IHlzH
Lean Six Sigma Green Belt Certification – Level II
Project Management Institute
AbesNmfFMpp7bWXv91fhBdMg-HqP
Operational Excellence Foundations
University of Washington
Biotechnology Project Management
University of Washington
Supervisor Orientation- professional and organizational development
The National Institutes of Health
HHSN27201201000024C
Good Clinical Practice(GCP)
The National Institutes of Health
Clinical Research Training certification by NIH(National Institute of Health)
Duke University
Data visualization & communication with Tableau(online)
https://www.linkedin.com/in/erey-montplaisir
Seattle, WA
Erey Montplaisir
NGS Quality Scientist at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/karlgrantuselman
Sammamish, WA
Grant Uselman
Quality at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/kellie-h-465aa132
Greater Seattle Area
Kellie H.
Sr. Research and Business Development Manager at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/marissa-vignali-27119312
Greater Seattle Area
Marissa Vignali
Associate Director, Client Data Management at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/charlessyi
Seattle, WA
Charles Yi, Ph.D.
Scientist II at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/gage-garcia
Seattle, WA
Gage Garcia M.S.
Interim QC Supervisor (Quality Control Associate II) at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/junhui-lucy-wang-51a54496
Seattle, WA
Junhui (Lucy) Wang
Senior Quality Control Associate at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/rebecca-nowicki-a1101950
Seattle, WA
Rebecca Nowicki
Laboratory General Supervisor  at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/kyle-jancola-4a7610161
Seattle, WA
Kyle Jancola
Research Associate II at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/jenniferdines
Seattle, WA
Jennifer Dines, MD
Medical Geneticist  |  Senior Medical Director at Adaptive Biotechnologies
https://www.linkedin.com/in/kthemarsh
Seattle, WA
Kevin Marshall
Research Associate 2 at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/cara-wilcox-a6452621
Redmond, WA
Cara Wilcox
Sr Manager, Regulatory Compliance at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/michael-dauer-72218372
Spokane-Coeur d'Alene Area
Michael Dauer
Associate Director, Technical Operations
https://www.linkedin.com/in/liz-beauregard
Seattle, WA
Liz Beauregard
Data Strategist
https://www.linkedin.com/in/maxwellsheffield
Seattle, WA
Maxwell Sheffield
Software Development Engineer In Test at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/sarah-weglein-1662b1191
Greater Seattle Area
Sarah Weglein
Area Supervisor at Adaptive Biotechnologies Corp.
https://www.linkedin.com/in/kristine-ann-buela
Greater Philadelphia
Kristine-Ann Buela
Skilled mucosal immunologist | Project management | Food and fitness enthusiast | Let’s connect!
https://www.linkedin.com/in/vishwas-bhatia-38733313
New York City Metropolitan Area
Vishwas Bhatia
Sr. Scientist at Thermo Fisher Scientific
Angana Deb
      
          



            
              
        
              
  

    
        Aanchal went above and beyond to make sure I was felt welcome when I first joined Adaptive and has since then always patiently answered my incessant questions regarding NGS. I'm so glad that as a fresher I have the privilege to learn from a person who has a wealth of knowledge in the field. I'm grateful for everything Aanchal does to help me grow.  Anahita Kiavand
      
          



            
              
        
              
  

    
        I had privilege of knowing Aanchal through my work at BBI. 

She has great knowledge in molecular biology/ NGS with pleasant personality. In fact, she encouraged me to take NGS courses with Ilumina that helped with my development too. 

She is an asset in the field ; I hope I can get a chance to work with her again.


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Today we are excited to announce a strategic partnership with Trivitron Healthcare, one of India's largest medical technology companies, to develop…
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Immuno-Oncology 360 attendees, mark your calendars for this panel presentation featuring Chief Business Development Officer Sharon Benzeno. She'll be…
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South Delhi
Aanchal Mehrotra
Account Executive at Vermmillion Communications
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Gurugram
Aanchal Mehrotra
Principal Associate Technology at WFX - World Fashion Exchange
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Mumbai
Aanchal Mehrotra
Mahindra Group | NMIMS University
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Mumbai
Aanchal Mehrotra
Animated Explainer Videos for Business & Services
Prasad Lab Members and Alumni
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Genomics: Next Generation DNA Sequencing (NGS) and Microarray
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Women In Bio - Seattle
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NGS (Next Generation Sequencing)
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Seattle AWIS
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