         ADJUNCT FACULTY, ZANVYL KREIGER SCHOOL OF ARTS AND SCIENCES, ADVANCED ACADEMIC PROGRAMS       Profile    Experienced Field Application Scientist with a unique balance of comprehensive, academic, wet-bench experience across all research sectors, a strategic understanding of scientific business including field marketing and technical sales, along with an ability to work alongside dynamic, intelligent teammates to close business for the team.      Areas of Expertise          Time and Territory Management   Molecular Biology Techniques   Assay Design/Development  Presentation Skills      DNA/RNA Sequencing  PCR  Cell Biology/Tissue Culture  Data Analysis/Software             Product Specialist/FAS Experience     February 2013   to   December 2015     Company Name   City  ,   State     Adjunct Faculty, Zanvyl Kreiger School of Arts and Sciences, Advanced Academic Programs        Co-Designed and Lectured for 12-14 graduate students attending the High Throughput Screening (HTS) and Automation Lab Course   Designed and executed 7 labs across the spectrum of detection chemistries commonly used in HTS labs throughout the semester   Provided lectures on the complex physical chemistry principles of all reagents used in the HTS labs Helped educate graduate students on the unique techniques used in an HTS lab as well as the unique instrumentation, software analysis and experimental designs used in these settings   Created quizzes, exams and a final comprehensive exam required for grading purposes as.         November 2005   to   Current     Company Name   City  ,   State     Senior Field Application Scientist/Product Specialist        Member of a regional sales team in our Discovery and Analytical Solutions division of PerkinElmer that includes 3 account managers across PA, DE, MD,DC, VA, NC and SC   Provide pre-sales and post-sales application support that will generate expected revenues in 2016 of $5.6 Million in the territory.  Facilitate relational sales through field support in diverse scientific sectors including: Pharmaceutical, Industrial/Biotech, Government, Military, Academic and Pre-Clinical Labs   Initiate scientific discussions to introduce and simplify PerkinElmer's high throughput screening reagents, consumables, multi-modal fluorescent plate readers and analysis software for complex small molecule, large molecule, basic biology and liquid-handling-aided, assay development projects   Interact with users of PerkinElmer's detection chemistries to disseminate technical education to our researcher community through various platforms such as live seminars, webinars, conference calls and individual protocol reviews.  Collaborate with scientists on the bench for comprehensive, proof-of-concept, wet lab demonstrations of our Alpha, LANCE, DELFIA and Luminescence chemistries in 96, 384 or 1536 well plates in our multimodal plate readers           Advise users on the proper experimental (assay) design, provide valuable expertise in constructing matrices for assay development, help construct efficient protocol workflows and assist with rational data analysis for conclusive results or necessary troubleshooting next steps   Design and execute offsite, internal, applications and instrument trainings for Sales Specialist as well as FAS   Gather and organize customer feedback from the field for distribution among the appropriate internal organizations such as Field Marketing, Strategic Marketing, R & D, Reagent Manufacturing and Instrument Manufacturing   Acted on specific customer feedback and applied my background in molecular biology, virology, cell biology and immunology to work with R & D internally to troubleshoot a field issue, design an assay or push a commercial kit idea to provide users with more relevant reagent solutions based on our detection chemistries.         February 2000   to   November 2005     Company Name   City  ,   State     PCR/SDS/Genomic Assays/SPS Technical Application Specialist        Provide technical recommendations on applicable Applied Biosystems product lines to internal and external customers through several different formats, telephone, email, voice-mail, or in person   Act as a liaison between the product group, sales, service and our customers to add value to our offerings through pre-sales, post-sales, service calls and internal interactions   Maintain leadership role in managing and communicating Sample Preparation Systems information to our TAS group   Conduct training seminars on Sample Preparation Systems (SPS), PCR, SDS and Real Time instrumentation, chemistry and theory for education of the entire AB Support Organization, Sales and external customers   Contribute concise sales trainings/support, small staff trainings, and internal colleague interactions to preserve the continuity of the AB message across the organization   Create and edit support documentation, FAQs, tutorials and instructions as help for customers using AB products, resources and theories   Train new hires, with critical introductory information, necessary for the knowledge base of a strong TAS.  Help to update colleagues with new product or theoretical information as we constantly expand our knowledge base Other Responsibilities: Lab Monitor (trainings/Demos), Lab Safety Officer, Administrative duties for TAS group.         August 1995   to   February 2000     Company Name   City  ,   State     Biologist        Constructed a full-length consensus clone of GB Virus B (GBV-B), an animal virus genetically and clinically related to the human Hepatitis C Virus Used nested RT-PCR to obtain viral cDNA from a serum pool chosen as the GBV-B cloning source.  Discovered novel sequence at the 3' end of the GBV-B genome by a combination of 5' RACE and RNA-ligase mediated RNA circularization PCR methods and proved that it was critical for infection.  Evaluated viral titers in vivo by nested RT-PCR (5).  Constructed an infectious GBV-      B clone, pGBB.  Contributed analyzed RT-PCR data to support the current argument that HGV (or GBV-C) was not a hepatitis virus with clinically relevant disease.  Discovered valuable information about the viability of GB Virus A (GBV-A) and GB Virus B (GBV-B), monkey viruses, to serve as surrogate models for studying their relationship to HCV.  Performed computer analysis on RNA structures (in-house software) and DNA sequences, using GCG Wisconsin Package, (analysis software), for determining the 5' and 3' ends of the GBV-B genome.  Created written guidelines for standard laboratory procedures in a Clean Room environment.  Trained experienced technical personnel to use standard appropriate molecular protocols for contamination control and safety.  Developed professional relationships with internal and external vendors for cost effective procurement of critical supplies and perishable reagents.         July 1992   to   August 1995     Company Name   City  ,   State     Research Assistant II        Assembled HIV retroviral vectors for using common cloning methodologies.  Transfected cloned HIV DNA into mammalian cell lines testing HIV gene expression Tested RNA transcription and DNA integration by Northern blot and Southern blot.  Applied cell culture techniques to maintain and prepare cell lines for viral studies.  Analyzed specific transfected cell lines for syncytium formation as a marker of in vitro HIV infection Performed DNA sequence data analysis using an ABI 373 A automated sequencer Synthesized the necessary primers using the oligonucleotide synthesizer ABI 394 Analyzed data using DNA sequence analysis software as well as Sequencer software Contributed molecular biology assistance to scientists studying HIV and SIV infection.         March 1989   to   July 1992     Company Name   City  ,   State     Process Development Associate        Performed microbiological screening to elucidate proteins with commercial value.  Conducted chemiluminescent, spectrophotometric assays for protease activity to evaluate novel proteins for further processing.  Conducted checks for bacterial pH, aeration, glucose levels, and enzyme production Operated 10-1000 L fermenters for production of marketable biologicals.  Performed DNA sequencing analysis, gas chromatography, protein column chromatography, protein precipitation and enzyme assays as quality controls for biologicals produced in pilot scale.          Education     2002     Johns Hopkins University   City  ,   State         Biotechnology    Biotechnology       1988     East Carolina University   City  ,   State       Bachelor of Science  :   Biotechnology    Biotechnology        Interests    30 years of youth sports service including: Co-Director of K-2 league, Coaching (ages K- 8) Basketball, Baseball, Football Tee-Ball and Soccer
*Elected to Mother Seton Parish Council (June 2003) *Appointed to St. Paul's Parish Council (June 2012) *2nd Degree Member of Knights of Columbus
*Board Member of St. Ann's CYO      Affiliations     Member: SLAS (Society for Lab Automation and Screening)  Member: LRIG (Laboratory Robotics Interest Group)       Additional Information      Community Service (Washington Metropolitan Area): 17 years of youth sports service including: Co-Director of K-2 league, Coaching (ages K- 8) Basketball, Baseball, Tee-Ball and Soccer Elected to Mother Seton Parish Council (June 2003) 2nd Degree Member of Knights of Columbus Board Member of St. Ann's CYO         Skills    Virology, molecular biology, cell culture techniques, chemistry, data analysis, DNA sequencing, scientific writing, molecular biology techniques, Real Time PCR, RT-PCR,  sample preparation, assay design, assay development,  assay troubleshooting, scientific presentations, seminars, strategic marketing, leadership, sales, time and territory management   