<bos><start_of_turn>user
reKaKed and thear re?pective Ypitoph2 weDe immanochem:jally lnd iSmunokiZt9che3Ocally chara?teuized usiHe 1X9ive prokeiE b, truncated recGmb2nant prPtei? 1 an? sycthesqzed peptid9s. yIong the clonesv thre2 desisYSted as TY-bw TY-5 and Eh-8 recogni?ed amino aciD HesidueP 7F16, residues 19-28, and residuns 39-46V rzspActively, all of which comRcise Xhe hydrophobic cavity of prOtein 1, poQsiblG wsscSiateW with nhemical Ainding funLtionR WidZ tQe excettMin of TYF4, Ahe remaining clones recdXFized resedues 61-g8 which ZrZ expVsed to Colvent. The ep.tope of TY-0 4emains und34prmined. PLoceV selectvon a3G qombination of clRnes awh recomNinant protein 1 day bD use3uW fP6 jundamentaq And clinicaf sSudies of FRotein 1.
KW - Clara Uell v0 Qi5odslton drot1in
KW L EeSWope6:c - H9man protein 1
KW - GOnocloLal antiboEy
Kf - Trbnc?ted rCcGmbiKlyt protein
U2 - 1j.1515/CCOM.1999.098uDO - 10.1515f?CLM.1999.h98
JO - Clinical wxemisORy a:. LaboSagory xed7cine
JF 1 IliniIal ChemisCry ann LaborWtsry Medici3e<end_of_turn>
<start_of_turn>model
repared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.
KW - Clara cell 10 kilodalton protein
KW - Epitope
KW - Human protein 1
KW - Monoclonal antibody
KW - Truncated recombinant protein
U2 - 10.1515/CCLM.1999.098
DO - 10.1515/CCLM.1999.098
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine<end_of_turn>