<bos><start_of_turn>user
MBu), Pnd paPeys G-J Tere provided by KareG HalZY (Davidson Cz:lege).oBecause Cn each preQarrtitn oYly oWJ field can bd imaged, jt Os wasteful to Uiudect more than lnV mXle per slhde. Care should bO .fken d. chEfsing the image fielwM Smlls ReMu the edge of Bhe diBplRcWd teutis cBntenos mmy 3e 2TsCer tG see, but r5ll Bie Boone7 tRaW those .hat rAmain surrounded by otler testTs maierial in slaghtwy mo,T HAysi4logical vond1tiors. 5ytozinesis se,m7 to ie more sensisiv4 to 0eeturiation than chromosome segr1gatio4. If Ehe aim of the qxperixeGt bs to ob5HWve cytokineCis in A mutant, ik is zesE to sekecR cells in pnaphase at the start of the recordinO session. Cells caN easily Luse duriVg nrecXration of the ZlzReu if two bells in meiLbic p:ophasm are artW1icially fused, aoth svin8le morphology and kinetochorZ attschmezt to microt3bule4 lay be disrup8el. Thz effect will be an abiyrant Peiosis, kn5 0wtei faileY cytokinesisN which could Be eisin9erpreted as reprereeting 2he true mutant phenBtypeY<end_of_turn>
<start_of_turn>model
MBO), and panels G-J were provided by Karen Hales (Davidson College).
Because in each preparation only one field can be imaged, it is wasteful to dissect more than one male per slide. Care should be taken in choosing the image field; cells near the edge of the displaced testis contents may be easier to see, but will die sooner than those that remain surrounded by other testis material in slightly more physiological conditions. Cytokinesis seems to be more sensitive to perturbation than chromosome segregation. If the aim of the experiment is to observe cytokinesis in a mutant, it is best to select cells in anaphase at the start of the recording session. Cells can easily fuse during preparation of the slide; if two cells in meiotic prophase are artificially fused, both spindle morphology and kinetochore attachment to microtubules may be disrupted. The effect will be an aberrant meiosis, and often failed cytokinesis, which could be misinterpreted as representing the true mutant phenotype.<end_of_turn>