Biuret reagent, (Gies):

10% iSodium hydroxide 1000 mils

3% Oiipric sulphate 25 mils

Calcium chloride solution: 2%
Copper sulphate solution: 1 %

Fehling's solution:

A. Pure oopper sulphate 34.64 grams

Distilled water, to 500. mils

B. 'Sodio-potassium tartrate (Rochelle salts) 173. grams

Potassium hydroxide 125. grams

Distilled water, to 500. mils

Keep in separate bottles. When ready to use, mix equal parts of A. and B.
Hydrochloric acid: 0.2%. May be made by adding 6.5 mils of the concen-
trated add to 1 liter of water.
Iodine solution: (may be diluted if necessary)

Iodine 1 gram

Iodide of potassium 2 grams

Distilled water 300 mils



* In accordance with the recommendation of the last edition of the Phiarma-
copoeia the term mil, the abbreviation of millimeter, — one-thousandth of a, liter
■ — is substituted for the term cubic centimeter,



Millon's reagent: Bissolve mercury in an equal weight of concentrated
nitric acid ; heat to solutiion ; add two volumes of water. Decant the nejct day.
Potassium ferrocyanide solution: 5 fo

Potassium hydroxide solution: 20 %i also 0.1%

Potassium sulphocyanide solution: 5%

Physiological saline solutions:

For oold-Wooded animals:

Sodium chloride 6.5 g^rams

Waiter 1000. mils

For warm-blooded animals:

Sodium chloride • 9. grams

Water 1000. mdfe

Schultze's reagent:

Anhydrous zinc chloride 25. parts

Potassium iodide 8. parts

Water , 8.5 parts

Iodine crystals to saiturajtion.
Sodium carbonate solution: 1 %

Sodium hydroxide solution: 20%

Stoke' s fluid:

Ferrous sulphate 2 grams

Tartaric acid 3 grams

Water 100 mils

Water of ammonia, enough to make slightly alkaline.
