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Digestive Enzymes at Work Laboratory Kits

By: The Flinn Staff

With the Digestive Enzymes at Work Biochemistry and Physiology Laboratory Kit, explore the roles of enzymes and the chemical reactions involved In the human digestive system.

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Explore the roles of enzymes and the chemical reactions involved in the human digestive system. The lab starts with amylase, which catalyzes the initial breakdown of starch molecules in the mouth. Students then observe the reaction of the enzyme pepsin with protein molecules in an acid environment, just like in the stomach. Finally, students analyze the “digestion” of milk by lipase, which catalyzed the hydrolysis of milk fat into fatty acids in the small intestine. Iodine, Benedict's reagent, biuret test solution, and litmus are included in the kit, along with the enzymes to test for the end products of digestion. Complete for 30 students working in pairs. Super Value Kit is complete for 5 classes of 30 students working in pairs. Boiling water baths are required for the Benedict's test.

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence

Disciplinary Core Ideas

MS-LS1.A: Structure and Function
MS-LS1.C: Organization for Matter and Energy Flow in Organisms
HS-LS1.A: Structure and Function
HS-LS1.C: Organization for Matter and Energy Flow in Organisms

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity
Systems and system models
Energy and matter
Structure and function

Performance Expectations

MS-LS1-3. Use argument supported by evidence for how the body is a system of interacting subsystems composed of groups of cells.
MS-LS1-7. Develop a model to describe how food is rearranged through chemical reactions forming new molecules that support growth and/or release energy as this matter moves through an organism
HS-LS1-2. Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.
HS-LS1-3. Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis.
HS-LS1-7. Use a model to illustrate that cellular respiration is a chemical process whereby the bonds of food molecules and oxygen molecules are broken and the bonds in new compounds are formed, resulting in a net transfer of energy.