Octopus got your tongue?

January 7, 2014 by Richard Hoops in Biology / Cell & Microbiology
Octopus Got Your Tongue?


The work is funded by a three-year grant from the National Science Foundation, and the investigators will use facilities at the Wrigley Marine Science Center on Catalina Island for some of their research.

The research is funded through an NSF program called INSPIRE (Integrated NSF Support Promoting Interdisciplinary Research and Education) that "is intended to encourage investigators to submit bold, exceptional proposals." The project at USC Dornsife certainly fits that description.

"Most people are surprised by this combination of linguistics and biology," said Khalil Iskarous, assistant professor of linguistics and a principal investigator on the NSF grant.

The project will compare the movement of the tongue to the movements of the arms of the and the body of a very small and thoroughly studied worm known as C. elegans. All three muscular systems work without bones that function as rigid "levers." Hydrostatic, their function is based on the resistance of fluids to compression. Squeezing water in one part of a muscle exerts pressure on water in other parts of the muscle forcing it to move.

"The hydrostatic principle is the common denominator for these three systems," Iskarous said. "For the worm, it's for locomotion; for the octopus, it's for manipulation; for the tongue, it's for communication and speech."

Iskarous will work with a USC Wrigley Institute faculty member, Andrew Gracey, associate professor of biological sciences and a co-principal investigator on the grant. Gracey will examine the nematode worm C. elegans and the connection between the worm's proteins and its neurons to determine the connection between its genetics and its behavior. A third researcher on the project—the lead octopus expert—is Jennifer Mather, professor of psychology at the University of Lethbridge in Alberta, Canada.

The Worm

C. elegans has served as an "experimental workhorse" since the 1970s, and it was used in genetic research that led to the Nobel Prize in Physiology or Medicine in 2002. (One of the Nobel laureates, Sydney Brenner, honored C. elegans as "Nature's Gift to Science" in the title of his Nobel lecture.) C. elegans is a nematode, part of a phylum of worms found in nearly every ecosystem, from the tropics to the poles, from the lowest elevation to the highest, and it measures one millimeter end-to-end.

Iskarous described C. elegans as a "simple complex organism," adding that decades of research have documented the characteristics of every one of its cells.

"We know the number of neurons in C. elegans; we know the connectivity between every neuron and every other neuron, and we know which neuron cells are connected to muscle cells," Iskarous said. "If you know the neural layout and the neuromuscular layout, you have a chance at formulating simple mathematical ideas that are linked to movements of the organism and can be measured."

The NSF project will develop algorithms and geometric analyses to create "numerical descriptors" for movements of C. elegans under a variety of conditions, including mutations to its genes. Gracey said gene mutations often have obvious impacts on the worm's movements and coordination, and the numerical descriptors might be used to quantify the effects of different genes in C. elegans on movement. The same process might be used to describe the role of genetics in the movement of octopus arms and the human tongue.

Octopus Got Your Tongue?


The Octopus

In the search for rules that govern hydrostatic muscular systems, the project will examine one of the most versatile systems of all—the arm of the octopus. Each of its eight limbs can adopt so many configurations and perform so many manipulations that it seems to have "infinite degrees of freedom," Mather said.


Mather said each octopus arm has extraordinarily complex sets of muscles, and the animal can use some of them as a "skeleton" for support while other muscles contract.

"An octopus can extend or contract its arm, bend it anywhere along its length and twist as much as it likes," Mather said. "Theoretically, it can make a knot in its arm and run the knot down its length and off the tip.

"But there must be rules for how they can move," Mather continued. "For example, there must be different rules to run a muscle system that has no fixed skeleton, like the octopus, from the rules that run for a muscle system with a fixed skeleton."

Mather is an ethologist—an expert in animal behavior—and she has been studying and writing about the octopus and other cephalopods since 1978. She will help Iskarous and Gracey search for similarities between the octopus' manipulation of its arms with the movement and function of the human tongue.



"We hope to base most of our study of their natural behavior on field observations using scuba diving, snorkeling and video recordings by collaborators," Alupay said.

The Tongue

Iskarous has studied how the tongue is affected by Parkinson's disease as well as how tongue movements differ according to influences of age, dialect and language. (Two years ago, he studied speech by young French-speakers in Montreal as a Distinguished Fulbright Visiting Chair at McGill University.)

"The human tongue is a very different muscular system than the rest of the human body," Iskarous said. "Our bodies are vertebrate mechanisms that operate by muscle working on bone to move. The tongue is in a different muscular family, much like an invertebrate. It's entirely muscle—it's muscle moving muscle."


The NSF research will explore the promise of C. elegans as a model for the dysarthria caused by Parkinson's disease. Dopamine synthesis and uptake in C. elegans will be manipulated to model the abnormal dopamine signaling in people with Parkinson's disease.

"The degeneration of cells that produce this chemical neurotransmitter, dopamine, have effects on behavior," Iskarous said. "C. elegans doesn't have a brain or basal ganglia, but some of its cells use dopamine in a way similar to mammals. With the worms, we can manipulate dopamine in much finer ways than is possible in more complex organisms, and we may have a chance to understand more complex connectivity in an animal like an octopus or a human being."

Finding a Common Language


"Linguists have a way of describing speech," Gracey said. "They have a nomenclature, a system of annotation for describing speech—how the tongue moves, how the palate moves, how the lips move—so when they describe the way different sounds are made, they have a standard vocabulary. There isn't a similar structured vocabulary for octopus movements. In addition, we're trying to find a mathematical way to describe the worm's movement which we can then directly apply to these other movements by the octopus arm and the human tongue.

"It's a real challenge to compare multiple systems, and this has been a challenge in biology for a long time," Gracey said. "You don't have to do this if you're going to work on one organism, but if you're working on more than one organism you need to be speaking the same language and be using the same types of descriptors. We're still scratching the surface on this—it's a very complicated project."

Provided by University of Southern California

"Octopus got your tongue?" January 7, 2014 https://phys.org/news/2014-01-octopus-tongue.html