Engineers pave way to 'artificial nose'

September 29, 2008 By Anne Trafton


The work could also allow scientists to unlock the mystery of how the sense of smell can recognize a seemingly infinite range of odors.

"Smell is perhaps one of the oldest and most primitive senses, but nobody really understands how it works. It still remains a tantalizing enigma," said Shuguang Zhang, associate director of MIT's Center for Biomedical Engineering and senior author of a paper on the work appearing online this week in the Proceedings of the National Academy of Sciences (PNAS).

Artificial noses could one day replace drug- and explosive-sniffing dogs, and could have numerous medical applications, according to Zhang and his colleagues. DARPA recently approved funding for the team's MIT (microfluidic-integrated transduction) RealNose project.

Until now, efforts to understand the molecular basis of smell have been stymied by the difficulty in working with the proteins that detect odors, known as olfactory receptors.


Smell is one of the most complex and least-understood senses. Humans have a vast olfactory system that includes close to 400 functional genes, more than are dedicated to any other function. Animals such as dogs and mice have around 1,000 functional olfactory receptor genes.

That variety of receptors allows humans and animals to discern tens of thousands of distinct odors. Each odor activates multiple receptors and this pattern of activation creates a signature that the brain can recognize as a particular scent.

The olfactory receptors that bind to odor molecules are membrane proteins, which span the cell surface. Since cell membranes are composed of a bilayer of fatty lipid molecules, the receptor proteins are highly hydrophobic (water-fearing).

When such proteins are removed from the cell and placed in water-based solutions, they clump up and lose their structure, said Liselotte Kaiser, lead author of the PNAS paper. That makes it very difficult to isolate the proteins in quantities large enough to study them in detail.

Kaiser and others spent several years developing a method to isolate and purify the proteins by performing each step in a hydrophobic detergent solution, which allows the proteins to maintain their structure and function.

The technique reported this week in PNAS involves a cell-free synthesis using commercially available wheat germ extract to produce a particular receptor, then isolating the protein through several purification steps. The method can rapidly produce large amounts of protein — enough to start structural and functional studies.

The team has also demonstrated a similar method that uses engineered mammalian cells to produce the receptors. That method, reported in PLoS One in August, takes more time and labor than the cell-free approach, but could have advantages in that the receptor is processed more naturally.


Source: Massachusetts Institute of Technology

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